Authors:
Anders Dahl
;
Thomas Martini Jørgensen
;
Phanindra Gundu
and
Rasmus Larsen
Affiliation:
Technical University of Denmark, Denmark
Keyword(s):
Particle analysis, Deconvolution, Depth estimation, Microscopic imaging.
Related
Ontology
Subjects/Areas/Topics:
Computer Vision, Visualization and Computer Graphics
;
Enhancement and Restoration
;
Feature Extraction
;
Features Extraction
;
Illumination and Reflectance Modeling
;
Image and Video Analysis
;
Image Formation and Preprocessing
;
Informatics in Control, Automation and Robotics
;
Segmentation and Grouping
;
Signal Processing, Sensors, Systems Modeling and Control
;
Statistical Approach
Abstract:
Process optimization often depends on the correct estimation of particle size, their shape and their concentration. In case of the backlight microscopic system, which we investigate here, particle images suffer from out-of-focus blur. This gives a bias towards overestimating the particle size when particles are behind or in front of the focus plane. In most applications only in-focus particles get analyzed, but this weakens the statistical basis and requires either particle sampling over longer time or results in uncertain predictions. We propose a new method for estimating the size and the shape of the particles, which includes out-of-focus particles. We employ particle simulations for training an inference model predicting the true size of particles from image observations. This also provides depth information, which can be used in concentration predictions. Our model shows promising results on real data with ground truth depth, shape and size information. The outcome of our approa
ch is a reliable particle analysis obtained from shorter sampling time.
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