An Experimental Platform Aimed at Long Lasting Electrophysiological Multichannel Recordings of Neuronal Cultures

G. Regalia; E. Biffi; A. Lucchini; M. Capriata; S. Achilli; A. Menegon; G. Ferrigno; L. Colombo; A. Pedrocchi

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Paper

An Experimental Platform Aimed at Long Lasting Electrophysiological Multichannel Recordings of Neuronal Cultures

In Proceedings of the 5th International Joint Conference on Computational Intelligence - Volume 1: SSCN, 551-557, 2013 , Vilamoura, Algarve, Portugal

Authors: G. Regalia ¹ ; E. Biffi ¹ ; A. Lucchini ² ; M. Capriata ¹ ; S. Achilli ¹ ; A. Menegon ³ ; G. Ferrigno ¹ ; L. Colombo ² and A. Pedrocchi ¹

Affiliations: ¹ Politecnico di Milano, Italy ; ² Politecnico di Milano University, Italy ; ³ San Raffaele Institute, Italy

Keyword(s): Microelectrode Arrays, Neuronal Cultures, Long-term Recordings.

Abstract: The elucidation of physio-pathological mechanisms expressed by a neuronal network over extended time scales (i.e., from hours to weeks) is the goal of many neurophysiological and neuropharmacological in vitro studies. In this context, a challenging technological requirement is the establishment of an experimental setup able to collect long-term neuronal signals. In this work we report the development of a compact environmental chamber designed to perform prolonged recordings of the bioelectrical activity exhibited by neuronal networks grown on MicroElectrode Arrays (MEAs). To reproduce an environment suitable for cells growth (temperature, pH and humidity) the chamber was coupled with a temperature control system and an air humidifying module. Validation tests demonstrated that the environment inside the portable chamber is comparable to standard cell incubators environment. To collect neuronal extracellular signals, custom multichannel pre-processing boards have been developed and i ntegrated with the chamber. With this equipment, we were able to reliably record spontaneous neuronal electrical activity from hippocampal cultures grown inside the chamber for several hours, which is not possible with the standard MEA recording setup due to environmental fluctuations. This system can collect multichannel data from neuronal cultures over long periods, providing an effective solution for long-term studies of neural activity. (More)

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Paper citation in several formats:

Regalia, G.; Biffi, E.; Lucchini, A.; Capriata, M.; Achilli, S.; Menegon, A.; Ferrigno, G.; Colombo, L. and Pedrocchi, A. (2013). An Experimental Platform Aimed at Long Lasting Electrophysiological Multichannel Recordings of Neuronal Cultures. In Proceedings of the 5th International Joint Conference on Computational Intelligence (IJCCI 2013) - SSCN; ISBN 978-989-8565-77-8; ISSN 2184-3236, SciTePress, pages 551-557. DOI: 10.5220/0004659005510557

@conference{sscn13,
author={G. Regalia. and E. Biffi. and A. Lucchini. and M. Capriata. and S. Achilli. and A. Menegon. and G. Ferrigno. and L. Colombo. and A. Pedrocchi.},
title={An Experimental Platform Aimed at Long Lasting Electrophysiological Multichannel Recordings of Neuronal Cultures},
booktitle={Proceedings of the 5th International Joint Conference on Computational Intelligence (IJCCI 2013) - SSCN},
year={2013},
pages={551-557},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0004659005510557},
isbn={978-989-8565-77-8},
issn={2184-3236},
}

TY - CONF

JO - Proceedings of the 5th International Joint Conference on Computational Intelligence (IJCCI 2013) - SSCN
TI - An Experimental Platform Aimed at Long Lasting Electrophysiological Multichannel Recordings of Neuronal Cultures
SN - 978-989-8565-77-8
IS - 2184-3236
AU - Regalia, G.
AU - Biffi, E.
AU - Lucchini, A.
AU - Capriata, M.
AU - Achilli, S.
AU - Menegon, A.
AU - Ferrigno, G.
AU - Colombo, L.
AU - Pedrocchi, A.
PY - 2013
SP - 551
EP - 557
DO - 10.5220/0004659005510557
PB - SciTePress