Study on the Fidelity of Biodevice T7 DNA Polymerase

Ming Li; Zhong-Can Ou-Yang; Yao-Gen Shu; Yao-Gen Shu

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Paper

Study on the Fidelity of Biodevice T7 DNA Polymerase

Topics: Biostatistics and Stochastic Models; Simulation and Modeling

In Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 3: BIOSTEC, 135-139, 2018 , Funchal, Madeira, Portugal

Authors: Ming Li ¹ ; Zhong-Can Ou-Yang ² and Yao-Gen Shu ^{1

;

2}

Affiliations: ¹ University of Chinese Academy of Sciences, China ; ² Institute of Theoretical Physics, Chinese Academy of Sciences, China

Keyword(s): Biodevice, DNA Polymerase, Fidelity, 1st-order Terminal Effects.

Abstract: We proposed a comprehensive kinetic model of steady-state copolymerization and obtain analytical solution of the high replication fidelity of the biodevice DNA polymerase. Our analytical calculations definitively show that the neighbor effects are the key factor of the overall fidelity. These analytical results were further demonstrated by T7 DNAp whose fidelity (106) is well described by the 1st-order neighbor effect.

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Paper citation in several formats:

Li, M.; Ou-Yang, Z. and Shu, Y. (2018). Study on the Fidelity of Biodevice T7 DNA Polymerase. In Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2018) - BIOINFORMATICS; ISBN 978-989-758-280-6; ISSN 2184-4305, SciTePress, pages 135-139. DOI: 10.5220/0006636101350139

@conference{bioinformatics18,
author={Ming Li. and Zhong{-}Can Ou{-}Yang. and Yao{-}Gen Shu.},
title={Study on the Fidelity of Biodevice T7 DNA Polymerase},
booktitle={Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2018) - BIOINFORMATICS},
year={2018},
pages={135-139},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0006636101350139},
isbn={978-989-758-280-6},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2018) - BIOINFORMATICS
TI - Study on the Fidelity of Biodevice T7 DNA Polymerase
SN - 978-989-758-280-6
IS - 2184-4305
AU - Li, M.
AU - Ou-Yang, Z.
AU - Shu, Y.
PY - 2018
SP - 135
EP - 139
DO - 10.5220/0006636101350139
PB - SciTePress