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Author: Evgeny O. Puchkov

Affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Federation

ISBN: 978-989-8425-36-2

Keyword(s): Computer image analysis, Fluorescence microscopy, Yeast culture viability, Brownian motion, Intracellular viscosity, Nucleic acid intercalator, Anticancer drug.

Related Ontology Subjects/Areas/Topics: Bioinformatics ; Biomedical Engineering ; Image Analysis

Abstract: For Saccharomyces cerevisiae yeast studies, three approaches have been developed. They are based on the image analysis (ImageJ software) application for the fluorescence microscopy data treatment. The first is a computer-aided fluorescence microscopy procedure for quantifying of the damaged cells in the ethanol-producing yeast culture. It was shown to be applicable for the assessment of the culture viability. The second is a means of characterizing Brownian motion of the insoluble polyphosphate complexes in the vacuoles. Using this approach, the apparent viscosity in the vacuoles was measured. The third is a method for locating intracellular sites/targets of the nucleic acid intercalators. This method may be of help in designing of new DNA-targeted drugs and in preliminary studies of their interaction with eukaryotic cells.

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Paper citation in several formats:
O. Puchkov, E. (2011). IMAGE ANALYSIS COMBINED FLUORESCENCE MICROSCOPY - Examples of ImageJ Software Application in Yeast Studies.In Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011) ISBN 978-989-8425-36-2, pages 258-261. DOI: 10.5220/0003120902580261

@conference{bioinformatics11,
author={Evgeny O. Puchkov.},
title={IMAGE ANALYSIS COMBINED FLUORESCENCE MICROSCOPY - Examples of ImageJ Software Application in Yeast Studies},
booktitle={Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011)},
year={2011},
pages={258-261},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003120902580261},
isbn={978-989-8425-36-2},
}

TY - CONF

JO - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011)
TI - IMAGE ANALYSIS COMBINED FLUORESCENCE MICROSCOPY - Examples of ImageJ Software Application in Yeast Studies
SN - 978-989-8425-36-2
AU - O. Puchkov, E.
PY - 2011
SP - 258
EP - 261
DO - 10.5220/0003120902580261

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