Authors:
Ardyan Pradana Putra
1
;
Hartono Kahar
2
and
Ahmad Yudianto
3
Affiliations:
1
Master of Forensic Science School Postgraduate School, Universitas Airlangga, Surabaya and Indonesia, Indonesia
;
2
Department Clinical Pathology Faculty of Medicine Universitas Airlangga Surabaya, Indonesia
;
3
Master of Forensic Science School Postgraduate School, Universitas Airlangga, Surabaya, Indonesia, Department Forensic Medicine and Medicolegal, Faculty of Medicine, Universitas Airlangga, Surabaya and Indonesia, Indonesia
Keyword(s):
Agglutination level, bloodstain, forensic identification, protein quantity, washing
Abstract:
Forensic examination of bloodstain provides important information to uncover inquired offense because blood is easily scattered in almost all forms of violence, and its biological state has specific properties in each person. In reality, perpetrators often attempt to conceal blood stained evidences to block revelation during investigation by disposing, burying, burning & washing. A number of stained evidences account but worn clothes during the incident are usually treated in either of the form, and when washed, chemicals such as detergents and bleach solution (NaClO) are used. The purpose of this study was to investigate the effect of washing on protein quantity and agglutination level on bloodstain in clothes for forensic identification. This is a laboratory experimental study, in which 32 samples of bloodstained clothes were given different treatments: tap water only, detergents and bleach solution with a control without washing. From the total of 32 samples, 16 samples were measu
red for protein quantity using UV spectrophotometer and 16 samples for agglutination level. The results were then analyzed using statistical parametric One Way Anova with significance level of 0.05. The result of statistical test obtained (p value <0.05) showing that there was a difference between the mean of protein quantity and agglutination level on bloodstain sample in each treatment. This research concluded that there is an effect on blood protein quantity and agglutination level due to washing type used (tap water, detergent, and bleach solution) by its decreasing trend supported by leaching and protein denaturation behavior.
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