Authors:
Imam Agus Faizal
1
;
Agung Dwi Wahyu Widodo
2
and
Jusak Nugraha
3
Affiliations:
1
Post Graduate Master of Immunology, Sekolah Pascasarjana, Universitas Airlangga, Surabaya, Department of Stem Cell Institute of Tropical Disease and Universitas Airlangga, Indonesia
;
2
Department of Microbiology Clinic, Faculty of Medicine and Dr. Soetomo Hospital, Indonesia
;
3
Department of Patology Clinic, Faculty of Medicine and Dr. Soetomo Hospital, Indonesia
Keyword(s):
Granuloma, Macrophage, Interleukin 6.
Abstract:
Granuloma is a pathological sign of host response as a system of defense against infection of Mycobacterium tuberculosis (Mtb) causing Tuberculosis (TB) disease in humans estimated to be 8.7 million new cases, 1.4 million deaths, and about 2 billion latent infections. Macrophages are responsible for activating protective immune responses in innate and adaptive immune to control or eliminate infection. The inner body protects against Mtb infection producing various secretions of cytokines interleukin 6 (IL-6) that play the role of activating multinucleated giant cells, differentiation of macrophage T cells and thus stimulating CD4+ and CD8+ T-cells to strengthen macrophage antimicrobial capacity as early response reactions (early phase). The aim of this research is to see the effect of dose of macrophage on secretion and IL-6 expression on a TB granuloma model in vitro. Human blood was made by Peripheral Blood Mononuclear Cell (PBMC) and treated with the addition of a dose of macroph
ages of 1x105 cells/well, 2x105 cells/well and 3x105 cells/well and control (without macrophages). Then the bacterium Mycobacterium tuberculosis was added and then observing the secretion The enzyme-linked immunosorbent assay (ELISA) method of IL-6 cytokine during the 1st, 2nd, 3rd, 4th, and 5th days. The results of IL-6 examination on ELISA obtained p value of 0.7520 (p> 0.05). The conclusion of the study was that there was no effect of adding macrophage dose to cytokine secretion of IL-6 levels on granuloma TB model in vitro.
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