loading
Documents

Research.Publish.Connect.

Paper

Paper Unlock

Authors: Marcelo Rodrigues 1 ; Cátia Correia 1 ; Célia M. Silveira 1 ; José J. G. Moura 1 ; Estibaliz Ochoteco 2 ; Elena Jubete 2 and M. Gabriela Almeida 3

Affiliations: 1 Universidade Nova de Lisboa, Portugal ; 2 CIDETEC-IK4 — Sensors and Photonics Unit, Spain ; 3 Universidade Nova de Lisboa and Escola Superior de Saúde Egas Moniz, Portugal

ISBN: 978-989-8425-91-1

Keyword(s): Nitrite, Cytochrome c nitrite reductase, Electrochemical biosensors, Screen printed electrodes.

Related Ontology Subjects/Areas/Topics: Biomedical Engineering ; Biomedical Instrumentation ; Biomedical Instruments and Devices ; Biomedical Sensors ; Devices ; Health Monitoring Devices ; Human-Computer Interaction ; Physiological Computing Systems

Abstract: This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40C or 60C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performan ce of these electrodes was satisfactory, with a sensitivity of 52 A.M-1.cm-2 within a linear range of 0.001 - 1 mM. (More)

PDF ImageFull Text

Download
Sign In Guest: Register as new SciTePress user now for free.

Sign In SciTePress user: please login.

PDF ImageMy Papers

You are not signed in, therefore limits apply to your IP address 18.206.241.26

In the current month:
Recent papers: 100 available of 100 total
2+ years older papers: 200 available of 200 total

Paper citation in several formats:
Rodrigues, M.; Correia, C.; M. Silveira, C.; J. G. Moura, J.; Ochoteco, E.; Jubete, E. and Gabriela Almeida, M. (2012). NITRITE BIOSENSING WITH DISPOSABLE ELECTRODE STRIPS - A Preliminary Study.In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012) ISBN 978-989-8425-91-1, pages 32-36. DOI: 10.5220/0003757700320036

@conference{biodevices12,
author={Marcelo Rodrigues. and Cátia Correia. and Célia M. Silveira. and José J. G. Moura. and Estibaliz Ochoteco. and Elena Jubete. and M. Gabriela Almeida.},
title={NITRITE BIOSENSING WITH DISPOSABLE ELECTRODE STRIPS - A Preliminary Study},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012)},
year={2012},
pages={32-36},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003757700320036},
isbn={978-989-8425-91-1},
}

TY - CONF

JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012)
TI - NITRITE BIOSENSING WITH DISPOSABLE ELECTRODE STRIPS - A Preliminary Study
SN - 978-989-8425-91-1
AU - Rodrigues, M.
AU - Correia, C.
AU - M. Silveira, C.
AU - J. G. Moura, J.
AU - Ochoteco, E.
AU - Jubete, E.
AU - Gabriela Almeida, M.
PY - 2012
SP - 32
EP - 36
DO - 10.5220/0003757700320036

Login or register to post comments.

Comments on this Paper: Be the first to review this paper.