Authors:
Marcelo Rodrigues
1
;
Cátia Correia
1
;
Célia M. Silveira
1
;
José J. G. Moura
1
;
Estibaliz Ochoteco
2
;
Elena Jubete
2
and
M. Gabriela Almeida
3
Affiliations:
1
Universidade Nova de Lisboa, Portugal
;
2
CIDETEC-IK4 — Sensors and Photonics Unit, Spain
;
3
Universidade Nova de Lisboa and Escola Superior de Saúde Egas Moniz, Portugal
Keyword(s):
Nitrite, Cytochrome c nitrite reductase, Electrochemical biosensors, Screen printed electrodes.
Related
Ontology
Subjects/Areas/Topics:
Biomedical Engineering
;
Biomedical Instrumentation
;
Biomedical Instruments and Devices
;
Biomedical Sensors
;
Devices
;
Health Monitoring Devices
;
Human-Computer Interaction
;
Physiological Computing Systems
Abstract:
This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40C or 60C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performa
nce of these electrodes was satisfactory, with a sensitivity of 52 A.M-1.cm-2 within a linear range of 0.001 - 1 mM.
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