Bioactivity of Soybean Tempeh against Diarrhea Associated Pathogen Is More Correlated with the Number of Total Bacteria than Specific Major Bacterial Phylum

T. E. Pramudito; E. G. A. Putri; E. G. A. Paluphi; G. Florencia; M. P. Gunawan; M. R. Pratiwi; Y. Yogiara

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Paper

Bioactivity of Soybean Tempeh against Diarrhea Associated Pathogen Is More Correlated with the Number of Total Bacteria than Specific Major Bacterial Phylum

In Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science ICE-TES - Volume 1, 320-327, 2021 , Bandung, Indonesia

Authors: T. E. Pramudito ; E. G. A. Putri ; E. G. A. Paluphi ; G. Florencia ; M. P. Gunawan ; M. R. Pratiwi and Y. Yogiara

Affiliation: Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jl. Raya Cisauk Lapan No.70, Tangerang, Banten 15345, Indonesia

Keyword(s): Soybean Tempeh, ETEC, Anti-diarrhea, RT-PCR, Yeast Agglutination Assay.

Abstract: Soybean tempeh can reduce the severity of diarrhoea through the inhibition of enterotoxigenic Escherichia coli (ETEC) adhesion to intestinal cells. This bioactivity is due to the presence of bioactive oligosaccharides derived from degradation of soybean matrix by fungi. Tempeh also contains other microorganisms such as bacteria and there has been no report whether bacteria can also influence the anti-adhesion bioactivity of tempeh extract against ETEC. In this research, we quantified bacterial population in tempeh samples using real time polymerase chain reaction (RT-PCR) method and measured the anti-adhesion bioactivity against ETEC of the extract using yeast agglutination assay. Data from both analyses were compared to see if there is any correlation between the two variables. Bacterial quantification with RT-PCR was focused on the enumeration of total bacteria and two specific major bacterial phyla in tempeh: Firmicutes and γ-Proteobacteria. There was a significantly strong positi ve correlation (R = 0.733) between total number of bacteria with anti-adhesion bioactivity of tempeh. However, there was no strong correlation between the number of Firmicutes and y-Proteobacteria with anti-adhesion bioactivity. Our finding indicates that the anti-adhesion bioactivity of tempeh tends to increase following the abundance of bacteria but is not significantly affected by specific major bacterial phylum. (More)

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Paper citation in several formats:

Pramudito, T. E., Putri, E. G. A., Paluphi, E. G. A., Florencia, G., Gunawan, M. P., Pratiwi, M. R. and Yogiara, Y. (2022). Bioactivity of Soybean Tempeh against Diarrhea Associated Pathogen Is More Correlated with the Number of Total Bacteria than Specific Major Bacterial Phylum. In Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - ICE-TES; ISBN 978-989-758-601-9, SciTePress, pages 320-327. DOI: 10.5220/0010753500003113

@conference{ice-tes22,
author={T. E. Pramudito and E. G. A. Putri and E. G. A. Paluphi and G. Florencia and M. P. Gunawan and M. R. Pratiwi and Y. Yogiara},
title={Bioactivity of Soybean Tempeh against Diarrhea Associated Pathogen Is More Correlated with the Number of Total Bacteria than Specific Major Bacterial Phylum},
booktitle={Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - ICE-TES},
year={2022},
pages={320-327},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010753500003113},
isbn={978-989-758-601-9},
}

TY - CONF

JO - Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - ICE-TES
TI - Bioactivity of Soybean Tempeh against Diarrhea Associated Pathogen Is More Correlated with the Number of Total Bacteria than Specific Major Bacterial Phylum
SN - 978-989-758-601-9
AU - Pramudito, T.
AU - Putri, E.
AU - Paluphi, E.
AU - Florencia, G.
AU - Gunawan, M.
AU - Pratiwi, M.
AU - Yogiara, Y.
PY - 2022
SP - 320
EP - 327
DO - 10.5220/0010753500003113
PB - SciTePress