Authors:
Hongxia Ming
1
;
Shun Zhao
2
;
Jie Su
1
;
Yuan Jin
1
and
Jingfeng Fan
3
Affiliations:
1
Key Laboratory for Ecological Environment in Coastal Areas (SOA), National Marine Environmental Monitoring Center and Dalian, China
;
2
Key Laboratory for Ecological Environment in Coastal Areas (SOA), National Marine Environmental Monitoring Center, Dalian, College of Fisheries and Life Science, Dalian Ocean University and Dalian, China
;
3
Key Laboratory for Ecological Environment in Coastal Areas (SOA), National Marine Environmental Monitoring Center, Dalian, The Fourth Institute of Oceanography, SOA and Beihai, China
Keyword(s):
Sea surface microlayer, subsurface layer, bacterial abundance, bacterial community
Abstract:
Sea surface microlayers (SSM) are specific microbial habitats that are critical to global biochemical process. In this study, bacterial populations inhabiting the SSM from two types of coastal sites (nearshore vs. offshore) were investigated by culturing and DNA fingerprinting methods, and were compared with those of subsurface layer (SUB). Bacterial samples from the SSM were collected using a glass plate method during March, August, October and January in the Northern Yellow Sea. The total number of bacterial and culturable bacterial exhibited an obvious spatial distribution, with more abundant in the nearshore sites than the offshore sites, and they showed an obvious enrichment effect in the surface microlayer. DGGE and sequencing revealed a close relationship between the bacterial community in the surface microlayer and subsurface layer, with high similarities between the two dominant bacterial communities, Proteobacteria and Bacteroidetes. The bacterial abundance and diversity in
the surface microlayer are obviously superior to those in the subsurface layer. It is more likely for bacterial communities to show significant differences with spatial and temporal changes.
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