Authors:
Sha Zhao
1
;
Ya Wang
2
;
Beibei Bie
2
;
Le Xu
2
and
Hong Bai
2
Affiliations:
1
School of Medicine, Xi’an Pei hua University, Xi ’an, 710125, P.R. China , Genetics laboratory, College of life sciences, Northwest University, Xi’an, 710069 and P.R. China, China
;
2
School of Medicine, Xi’an Pei hua University, Xi ’an, 710125 and P.R. China, China
Keyword(s):
Microsatellite primer, Genetic diversity, Environmental protection
Abstract:
Termites are a kind of insect pest that is harmful to the environment. The genetic diversity of two natural populations in Xi'an and Nanjing was investigated using 6 pairs of highly polymorphic microsatellite markers. The results showed that the average number of alleles in Reticulitermes aculabialis populations of Xi'an and Nanjing was 2.075 and 2.011, respectively; the average number of effective alleles (Ne) was 1.747 and 1.705, respectively; and the average observed heterozygosity (Ho) was 0.483 and 0.445, respectively. The expected mean value of heterozygosity (He) was 0.366 and 0.344, respectively, while the average value of the diversity index (I) was 0.566 and 0.534, respectively. All the above results indicated that the genetic diversity and the degree of genetic variation within Xi'an and Nanjing populations were similar, both at a moderate but relatively low level. Additionally, the genetic structure of the populations in Xi 'an and Nanjing was analysed by GenALEx software
, showing that there was significant genetic differentiation (Fst=0.409) between the two populations, and the gene flow between the populations was relatively low (Nm = 0.375).
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