Authors:
Mariany Razali
1
;
Muhammad Taufik
2
;
Evi Dayatul Atiqah
3
;
Rid Wanto
3
;
Anny Sartika Daulay
3
;
Desi Ardilla
4
;
Endang Susilawati
5
and
Afniwati
5
Affiliations:
1
Pharmacy Department, Universitas Tjut Nyak Dhien, Medan, Indonesia, Indonesia
;
2
Chemistry Department, Faculty of Mathematic and Natural Science, Universitas Sumatera Utara, Medan 20155, Sumatera Utara, Indonesia, Indonesia
;
3
Pharmacy Department, UMN Al Washliyah, Medan, Indonesia, Indonesia
;
4
gricultural Technology Department, Universitas Muhammadiyah Sumatera Utara, Medan, Indonesia, Indonesia
;
5
Nurse Department, Poltekkes Kemenkes Medan, Indonesia
Keyword(s):
nicotine, cigarettes, electrosynthesis, paper chromatography, UV spectrophotometer
Abstract:
Nicotine is a substance contained in tobacco leaves which is used for the manufacture of cigarette raw materials. Nicotine will come out of tobacco in the process of smoking or inhaling second hand smoke. In cigarettes the nicotine content should not exceed 1.5 mg. Nicotine is very dangerous for health and is addictive. This study aims to determine the effect of tobacco weight on nicotine levels and to analyse the nicotine contained in commercial cigarettes by applying the paper chromatography method and UV-Vis spectrophotometry. In this study, an experimental method was used, the experimental method was developed on electro synthetic coupling maceration with variations in sample weights of 0.3, 0.6, 0.9, 0.12, and 0.15 grams. The qualitative test results obtained in the Paper Chromatography method were nicotine positive because the Rf value of the sample was close to the Rf value of the standard solution and was reddish yellow, using electro synthetic coupling maceration with quali
tative tests with Cyanogen bromide, the best results were marked by abundant greenish yellow colour . Using the UV-Vis spectrophotometry method, it was obtained that in each increase of 0.3 to 1.5 grams of tobacco, the concentration would increase by 0.4 ppm. Paper Chromatography Methods and UV-Vis Spectrophotometry can be used to analyse nicotine and produce the best results.
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