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Authors: Birgit Möller ; Oliver Greß ; Nadine Stöhr ; Stefan Hüttelmaier and Stefan Posch

Affiliation: Martin-Luther-University Halle-Wittenberg, Germany

ISBN: 978-989-674-029-0

Keyword(s): Active contours, Snakes, Wavelets, Segmentation, Fluorescent microscope images.

Related Ontology Subjects/Areas/Topics: Computer Vision, Visualization and Computer Graphics ; Feature Extraction ; Features Extraction ; Image and Video Analysis ; Informatics in Control, Automation and Robotics ; Segmentation and Grouping ; Signal Processing, Sensors, Systems Modeling and Control ; Wavelet Analysis

Abstract: Microscope imaging is an indispensable tool in modern systems biology. In combination with fully automatic image analysis it allows for valuable insights into biological processes on the sub-cellular level and fosters understanding of biological systems. In this paper we present two new techniques for automatic segmentation of cell areas and included sub-cellular particles. A new cascaded and intensity-adaptive segmentation scheme based on coupled active contours is used to segment cell areas. Structures on the sub-cellular level, i.e.~stress granules and processing bodies, are detected applying a scale-adaptive wavelet-based detection technique. Combining these results allows for complementary analysis of biological processes. It yields new insights into interactions between different particles and distributions of particles among different cells. Our experimental evaluations based on ground-truth data prove the high-quality of our segmentation results regardin g these aims and open perspectives towards deeper insights into biological systems on the sub-cellular level. (More)

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Paper citation in several formats:
Möller B.; Greß O.; Stöhr N.; Hüttelmaier S.; Posch S. and (2010). ADAPTIVE SEGMENTATION OF CELLS AND PARTICLES IN FLUORESCENT MICROSCOPE IMAGES.In Proceedings of the International Conference on Computer Vision Theory and Applications - Volume 2: VISAPP, (VISIGRAPP 2010) ISBN 978-989-674-029-0, pages 97-106. DOI: 10.5220/0002849100970106

@conference{visapp10,
author={Birgit Möller and Oliver Greß and Nadine Stöhr and Stefan Hüttelmaier and Stefan Posch},
title={ADAPTIVE SEGMENTATION OF CELLS AND PARTICLES IN FLUORESCENT MICROSCOPE IMAGES},
booktitle={Proceedings of the International Conference on Computer Vision Theory and Applications - Volume 2: VISAPP, (VISIGRAPP 2010)},
year={2010},
pages={97-106},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0002849100970106},
isbn={978-989-674-029-0},
}

TY - CONF

JO - Proceedings of the International Conference on Computer Vision Theory and Applications - Volume 2: VISAPP, (VISIGRAPP 2010)
TI - ADAPTIVE SEGMENTATION OF CELLS AND PARTICLES IN FLUORESCENT MICROSCOPE IMAGES
SN - 978-989-674-029-0
AU - Möller, B.
AU - Greß, O.
AU - Stöhr, N.
AU - Hüttelmaier, S.
AU - Posch, S.
PY - 2010
SP - 97
EP - 106
DO - 10.5220/0002849100970106

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