loading
Documents

Research.Publish.Connect.

Paper

Authors: Rina Purwati 1 ; Yoes Prijatna Dachlan 2 and Agung Dwi Wahyu Widodo 3

Affiliations: 1 Department of Immunology Postgraduate School, Airlangga University, Surabaya and East Jawa, Indonesia ; 2 Department of Anatomical Pathology, Faculty of Medicine, Airlangga University, Surabaya and East Jawa, Indonesia ; 3 Department of Clinical Microbiology, Faculty of Medicine, Airlangga University, Surabaya and East Jawa, Indonesia

ISBN: 978-989-758-348-3

Keyword(s): Apoptosis, Caspase-3, Eanterobacter cloacae, Proteus mirabilis

Abstract: Enterobacter cloacae and Proteus mirabilis are opportunistic pathogenic bacteria that are the main causes of high morbidity and mortality in humans from nosocomial infections in hospitals. Caspase-3 is a death protease that acts as an apoptotic execution on an intrinsic pathway. Caspase-3 plays an important role in the process of cell death that can divide various dead substrates often causing morphological and biochemical changes in cells. The increase in caspase-3 expression indicates the severity of the disease, DNA fragmentation, and condensation of apoptotic chromatin in the examined organs. This research type is true experimental using a post-test in only the control group. The aims of this study are to determine the expression of caspase-3 in the spleen and liver organs of experimental animals Rattus norvegicus, infected with E. cloacae and Proteus mirabilis. E. cloacae and P. mirabilis bacteria were injected through the rats’ peritoneum and then observed for 24 hours. The rats ’ spleens and livers were observed for caspase-3 expression with immunohistochemistry. Caspase-3 expression increases more in the liver than the spleen. The liver serves as a detoxification of infection by spending and inducing cleansing of bacterial infections. Excessive inflammatory responses to the liver can cause harmful effects on the host, with the cell cycle developing cellular stress, causing cell death through caspase (More)

PDF ImageFull Text

Download
CC BY-NC-ND 4.0

Sign In Guest: Register as new SciTePress user now for free.

Sign In SciTePress user: please login.

PDF ImageMy Papers

You are not signed in, therefore limits apply to your IP address 34.204.176.189

In the current month:
Recent papers: 100 available of 100 total
2+ years older papers: 200 available of 200 total

Paper citation in several formats:
Purwati, R.; Prijatna Dachlan, Y. and Dwi Wahyu Widodo, A. (2018). Different Expression of Caspase-3 in the Spleen and Liver of Rattus norvegicus Infected with Enterobacter cloacae and Proteus mirabilis as a Septic Model.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 269-273. DOI: 10.5220/0007541202690273

@conference{icps18,
author={Rina Purwati. and Yoes Prijatna Dachlan. and Agung Dwi Wahyu Widodo.},
title={Different Expression of Caspase-3 in the Spleen and Liver of Rattus norvegicus Infected with Enterobacter cloacae and Proteus mirabilis as a Septic Model},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={269-273},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007541202690273},
isbn={978-989-758-348-3},
}

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - Different Expression of Caspase-3 in the Spleen and Liver of Rattus norvegicus Infected with Enterobacter cloacae and Proteus mirabilis as a Septic Model
SN - 978-989-758-348-3
AU - Purwati, R.
AU - Prijatna Dachlan, Y.
AU - Dwi Wahyu Widodo, A.
PY - 2018
SP - 269
EP - 273
DO - 10.5220/0007541202690273

Login or register to post comments.

Comments on this Paper: Be the first to review this paper.