Authors:
Rina Purwati
1
;
Yoes Prijatna Dachlan
2
and
Agung Dwi Wahyu Widodo
3
Affiliations:
1
Department of Immunology Postgraduate School, Airlangga University, Surabaya and East Jawa, Indonesia
;
2
Department of Anatomical Pathology, Faculty of Medicine, Airlangga University, Surabaya and East Jawa, Indonesia
;
3
Department of Clinical Microbiology, Faculty of Medicine, Airlangga University, Surabaya and East Jawa, Indonesia
Keyword(s):
Apoptosis, Caspase-3, Eanterobacter cloacae, Proteus mirabilis
Abstract:
Enterobacter cloacae and Proteus mirabilis are opportunistic pathogenic bacteria that are the main causes of high morbidity and mortality in humans from nosocomial infections in hospitals. Caspase-3 is a death protease that acts as an apoptotic execution on an intrinsic pathway. Caspase-3 plays an important role in the process of cell death that can divide various dead substrates often causing morphological and biochemical changes in cells. The increase in caspase-3 expression indicates the severity of the disease, DNA fragmentation, and condensation of apoptotic chromatin in the examined organs. This research type is true experimental using a post-test in only the control group. The aims of this study are to determine the expression of caspase-3 in the spleen and liver organs of experimental animals Rattus norvegicus, infected with E. cloacae and Proteus mirabilis. E. cloacae and P. mirabilis bacteria were injected through the rats’ peritoneum and then observed for 24 hours. The rat
s’ spleens and livers were observed for caspase-3 expression with immunohistochemistry. Caspase-3 expression increases more in the liver than the spleen. The liver serves as a detoxification of infection by spending and inducing cleansing of bacterial infections. Excessive inflammatory responses to the liver can cause harmful effects on the host, with the cell cycle developing cellular stress, causing cell death through caspase
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