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Authors: Faroq AL-Tam 1 ; António dos Anjos 2 ; Stephane Bellafiore 3 and Hamid Reza Shahbazkia 4

Affiliations: 1 University of Algarve and University of Thamar, Portugal ; 2 ISMAT and University of Algarve, Portugal ; 3 IRD and LMI RICE, France ; 4 University of Algarve and IRD, Portugal

Keyword(s): Root Knot Nematodes, Vessel-like Detection, Illumination Correction, Thining, Mathematical Morphology.

Related Ontology Subjects/Areas/Topics: Bioimaging ; Biomedical Engineering ; Conventional Microscopy ; Feature Recognition and Extraction Methods ; Image Processing Methods ; Quantitative Bioimaging

Abstract: Object detection in microscopy image is essential for further analysis in many applications. However, images are not always easy to analyze due to uneven illumination and noise. In addition, objects may appear merged together with debris. This work presents a method for detecting rice root knot nematodes in microscopy images. The problem involves four subproblems which are dealt with separately. The uneven illumination is corrected via polynomial fitting. The nematodes are then highlighted using mathematical morphology. A binary image is obtained and the microscope lines are removed. Finally, the detected nematodes are counted after thresholding the non-nematode particles. The results obtained from the performed tests show that this is a reliable and effective method when compared to manual counting.

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Paper citation in several formats:
AL-Tam, F.; dos Anjos, A.; Bellafiore, S. and Shahbazkia, H. (2015). Detection of Root Knot Nematodes in Microscopy Images. In Proceedings of the International Conference on Bioimaging (BIOSTEC 2015) - BIOIMAGING; ISBN 978-989-758-072-7; ISSN 2184-4305, SciTePress, pages 76-81. DOI: 10.5220/0005209000760081

@conference{bioimaging15,
author={Faroq AL{-}Tam. and António {dos Anjos}. and Stephane Bellafiore. and Hamid Reza Shahbazkia.},
title={Detection of Root Knot Nematodes in Microscopy Images},
booktitle={Proceedings of the International Conference on Bioimaging (BIOSTEC 2015) - BIOIMAGING},
year={2015},
pages={76-81},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005209000760081},
isbn={978-989-758-072-7},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the International Conference on Bioimaging (BIOSTEC 2015) - BIOIMAGING
TI - Detection of Root Knot Nematodes in Microscopy Images
SN - 978-989-758-072-7
IS - 2184-4305
AU - AL-Tam, F.
AU - dos Anjos, A.
AU - Bellafiore, S.
AU - Shahbazkia, H.
PY - 2015
SP - 76
EP - 81
DO - 10.5220/0005209000760081
PB - SciTePress

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