Authors:
Jie He
;
Xiaoru Fan
;
Huan Liu
;
Yuan Liu
and
Haifeng Wei
Affiliation:
College of Marine Technology and Environment,Dalian Ocean University,Dalian, Key Laboratory of Nearshore Marine Environmental Science and Technology in Liaoning Province,Dalian Ocean University andDalian, China
Keyword(s):
Microbial remediation, Pseudomonas putida Y3 strain, crude oil in sediment, degradation
Abstract:
In this study, Pseudomonas putida Y3 strain was used as the test object, and the sediment contamination test was conducted at different crude oil concentrations (0, 4000, 8000, 12000, 16000 and 20000 mg/kg) in order to study the remediation effect of microorganisms on crude oil-contaminated sediments. The degradation rate of crude oil in sediment after the addition of Y3 strain was determined, and the degradation of crude oil content in contaminated sediment by Y3 strain was analyzed. The results showed that the growth of Y3 strain was affected by crude oil pollution, and the exogenous bacteria had an adaptive positive process to the sediment environment. After the microorganisms adjusted themselves, they were able to adapt to the new environment. The Y3 strain had an effect on the degradation of sediment crude oil. With the increase of sediment crude oil concentration, the degradation rate constant gradually increased. When the crude oil concentration was 12000 mg/kg, the rate const
ant of the experimental group was the highest (10.584). Studies have shown that Y3 strain has the remedial function for crude oil-contaminated sediments.
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