Authors:
Yan Fang
1
;
Xia Dong
1
;
Zhengjin Liu
2
and
Xiaoping Ying
1
Affiliations:
1
Laboratory of Molecular Pathology and Shaanxi University of Chinese Medicine, China
;
2
Zhongshan Hospital Xiamen University, China
Keyword(s):
Xenotrans plantation, hematoma cells, rejection.
Abstract:
Objective: To investigate if xenotransplantation of rabbit fracture hematoma cells in the broken ends of fractured bone can lead to obvious hyper acute and acute immune rejection. Methods: We conducted a trial involving 90 rabbits with 4~6 weeks old, weighting 2.2~2.6kg, male and female in half. They were randomly divided into three groups: fracture group (n=30), fracture transplantation normal saline group (n=30), and fracture transplantation hematoma group (n=30).3 days after animal models of sawed-off cubital bone was established, rabbits fracture hematoma cells which were derived from the ordinary livor blue rabbits’ fracture hematoma and cultured in vitro were transplanted into the broken ends of fractured bone. Ten rabbits were sacrificed in each group at the 1st, 4th, and 8th day after the transplantation. Immunohisto chemical method was adopted to observe the survival rate of fracture common chinchilla rabbit fracture hematoma cells, CD68+ macrophage infiltration and splenic
lymph follicles. Immunohisto chemical SABC method was used to detect the expression of CD68 in the macrophages of New Zealand white rabbits around the allograft. Results: After transplantation, the fracture hematoma cells of xenogeneic rabbits survived a lot at the fracture end, and the cells’ structure was normal. The splenic lymphoid follicles did not proliferate significantly in each group and there was no significant difference in the expression of CD68 macrophages at each interval between groups. Conclusion: There was no significant immunological rejection in the early stage of xenotransplantation of rabbit fracture hematoma cells in the broken ends of fracturedbone
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