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Authors: Tianyi Zhou ; Susan Perry and Svetlana Tatic-Lucic

Affiliation: Lehigh University, United States

ISBN: 978-989-758-071-0

Keyword(s): Viability, Hippocampal Neurons, Multi-Electrode Array (MEA), Dielectrophoresis (DEP), Sucrose, Membrane Potential.

Related Ontology Subjects/Areas/Topics: Biomedical Engineering ; Biomedical Instruments and Devices ; Biomedical Sensors ; Hardware ; MEMS ; Sensor Networks ; Technologies Evaluation

Abstract: In this work, we have investigated the viability of embryonic mouse hippocampal neurons after dielectrophoretic positioning on multi-electrode arrays (MEA). We present a systematic evaluation of positive dielectrophoretic conditions, including 1) an investigation of the effect of 10% sucrose (w/v in deionized water), a commonly used, low-conductivity buffer medium, on the viability of mouse hippocampal neurons over different time periods, and 2) a study of the effect of the membrane potential induced by DEP electric field on the integrity of the cell membrane. Post-DEP high neuronal viability was achieved experimentally, and spontaneous neuronal potentials from trapped neurons on the MEA were successfully recorded.

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Paper citation in several formats:
Zhou, T.; Perry, S. and Tatic-Lucic, S. (2015). On Combining the Dielectrophoresis and Microdevices - Investigation of Hippocampal Neuronal Viability after Implementing Dielectrophoretic Positioning on Multi-Electrode Arrays.In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2015) ISBN 978-989-758-071-0, pages 71-77. DOI: 10.5220/0005180200710077

@conference{biodevices15,
author={Tianyi Zhou. and Susan Perry. and Svetlana Tatic{-}Lucic.},
title={On Combining the Dielectrophoresis and Microdevices - Investigation of Hippocampal Neuronal Viability after Implementing Dielectrophoretic Positioning on Multi-Electrode Arrays},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2015)},
year={2015},
pages={71-77},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005180200710077},
isbn={978-989-758-071-0},
}

TY - CONF

JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2015)
TI - On Combining the Dielectrophoresis and Microdevices - Investigation of Hippocampal Neuronal Viability after Implementing Dielectrophoretic Positioning on Multi-Electrode Arrays
SN - 978-989-758-071-0
AU - Zhou, T.
AU - Perry, S.
AU - Tatic-Lucic, S.
PY - 2015
SP - 71
EP - 77
DO - 10.5220/0005180200710077

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