Authors:
Zahreni Hamzah
1
;
Suhartono Taat Putra
2
and
Elyana Asnar
3
Affiliations:
1
Universitas Jember, Indonesia
;
2
Department of Dental Biomedic Sciences, Faculty of Dentistry, University of Jember, Jember, Indonesia
;
3
Universitas Airlangga, Indonesia
Keyword(s):
Distress, Corticosterone, Hsp-60, TGF-β, Casp-3, PDLSC, Tooth mobility
Abstract:
Tooth mobility is often found in workers with chronic work distress. Tooth mobility initially with the surrounding periodontal tissue destruction may be due to stress hormones. Periodontal tissue turnover capability is determined by the ability of differentiation of periodontal ligament stem cells/PDLSC into mature cells necessary for tissue formation. This study aimed to explain tooth mobility that is often found in workers with chronic work distress. This study used male Rattus norvegicus of Sprague-Dawley strain aged 3 months as the worker model. Methods. This was an experimental laboratory study using a Randomized Separate pretest-posttest control group design. The rats were grouped into four groups: Pre-test (P), post-test, consisting of control group (K), psychological distress (SP), and psycho-physical distress group (SPF), each consisting of eight rats. A stressor was provided with a home-made electric foot shock generator for 30 minutes every day in a communication box durin
g 7 days. Examination of expression of Hsp-60, casp-3 and TGF-β in PDLSC was performed with IHC. Data was processed with multivariate analysis. Increased levels of corticosterone in the distressed condition increased the number of Hsp-60, Casp-3 and TGF-β expressing PDLSC. Increased expression of Hsp-60, Casp-3 and TGF-b in PDLSC in the psychological and psycho-physical work distressed condition may induce tooth mobility.
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