Authors:
Xuan Zhang
;
Guangxin Yang
;
Cong Kong
;
Xiaosheng Shen
;
Youqiong Cai
and
Dongmei Huang
Affiliation:
East China Sea Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture and Shanghai 200090, China
Keyword(s):
formaldehyde; squid; liquid chromatography; 2,4-dinitrophenylhydrazine
Abstract:
Objective for rapid detecting free formaldehyde (FA) in marine products, a method with high sensitivity and little interference is described. Background value of FA in marine products would supplement the scientific evaluation index system. Free FA is derivatised with 2,4-dinitrophenylhydrazine (DNPH) to form a chromophore for high-performance liquid chromatography (HPLC) detection. The formation of the DNPH FA derivative is shortened to 30 min. It shows good linear correlation between the peak areas and FA concentrations with a dynamic linear range of 0.3-25.0 mg/L, the limit of detection (LOD) is 0.2 mg/Land the limit of quantitation (LOQ) is 0.5 mg/L. The recovery range of free FA in spiked squid was 70%-78% with relative standard deviation (RSD) of 5%-10% (n=5). FA content is detected in 14 species of seafood comparing to the past analysis method, results show no significant difference, FA content of tuna, tuna, cod, Surf Calm and cuttlefish is more than 40 mg/kg. The average of
FA content in 28 species of packaging squid products is 14.7 mg/kg, ranging from 2.10-61.8 mg/kg. This method is simpler and easier to operate; it reduces the concentration of derivatives, shortens the reaction time, and is applicable to the determination of formaldehyde content in all kinds of seafood.
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