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Authors: Gustavo Lelis da Silva 1 ; Jorge Stolfi 1 and Helena Leitão 2

Affiliations: 1 State University of Campinas (UNICAMP), Campinas, SP, Brazil ; 2 Federal Fluminense University (UFF) Niteroi, RJ, Brazil

Keyword(s): Optical Microscopy, 3D Printing, Multi-focus Stereo, Photometric Stereo, Arduino.

Abstract: Multifocal microscopy is an established technique to combine several reflected-light microscopy images of an object, each with a limited depth of focus, into a single image that shows the whole object in focus. Photometric stereo is an independent technique to recover the third dimension of an opaque object, given several images taken from the same viewpoint with different illumination conditions. We describe a low-cost motorized positioning device for the automatic acquisition of multiple reflected-light low-power microscope images of an object for combined multifocal and photometric stereo capture of millimeter-scale objects. The device automatically varies the microscope-to-object distance and the light direction, under control of an Arduino microcontroller. The device consists of a supporting frame, a microscope holder with motorized vertical displacement, an illumination stage with multiple light sources, and control electronics. The mechanical and structural parts are largely 3 D-printed. The electronics is based on the Arduino microcontroller. The device is driven through an USB cable by a Python program that runs on a PC. (More)

CC BY-NC-ND 4.0

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Paper citation in several formats:
Lelis da Silva, G.; Stolfi, J. and Leitão, H. (2022). MUFF: A Low-cost Motorized Positioner Developed by Additive Manufacturing to Obtain Multi-focus Images. In Proceedings of the 10th International Conference on Photonics, Optics and Laser Technology - PHOTOPTICS, ISBN 978-989-758-554-8; ISSN 2184-4364, pages 144-151. DOI: 10.5220/0010913500003121

@conference{photoptics22,
author={Gustavo {Lelis da Silva}. and Jorge Stolfi. and Helena Leitão.},
title={MUFF: A Low-cost Motorized Positioner Developed by Additive Manufacturing to Obtain Multi-focus Images},
booktitle={Proceedings of the 10th International Conference on Photonics, Optics and Laser Technology - PHOTOPTICS,},
year={2022},
pages={144-151},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010913500003121},
isbn={978-989-758-554-8},
issn={2184-4364},
}

TY - CONF

JO - Proceedings of the 10th International Conference on Photonics, Optics and Laser Technology - PHOTOPTICS,
TI - MUFF: A Low-cost Motorized Positioner Developed by Additive Manufacturing to Obtain Multi-focus Images
SN - 978-989-758-554-8
IS - 2184-4364
AU - Lelis da Silva, G.
AU - Stolfi, J.
AU - Leitão, H.
PY - 2022
SP - 144
EP - 151
DO - 10.5220/0010913500003121