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Authors: Stefan Sokoll 1 ; Klaus Tönnies 2 and Martin Heine 1

Affiliations: 1 Leibniz Institute for Neurobiology, Germany ; 2 Otto-von-Guericke University, Germany

ISBN: 978-989-8425-36-2

Keyword(s): 3D particle localization, Nanometer sub-resolution, Live cell imaging, Confocal fluorescence microscopy.

Related Ontology Subjects/Areas/Topics: Algorithms and Software Tools ; Bioinformatics ; Biomedical Engineering ; Computational Molecular Systems ; Image Analysis ; Model Design and Evaluation ; Pattern Recognition, Clustering and Classification

Abstract: Studying molecular dynamics is crucial for understanding biological processes in living cells. In principle, this is achieved by attaching fluorescent particles to molecules of interest and their detection using fluorescence microscopy. These analysis require fast optical techniques with at least 20Hz frame rate and a resolution below the diffraction limit in all three spatial dimensions. Current approaches basically rely on determining the correlation between features of the particle’s 2D point spread function (PSF) and the focal distance to the center of the particle. However, they are still unsuitable for the application to live cell imaging where the refractive index mismatch is present. This mismatch leads to non-stationary optical properties of the particles on which the algorithms rely, necessitating a calibration procedure prior to every experiment. However, this is almost unfeasible to particles attached to living cells. We established a spinning disk confocal setup and emplo y Quantum dots (QD) as fluorescence particles. Corresponding models of the axial PSF features that define the distance to the center of the particle are developed and analyzed in the presence of the refractive index mismatch. We present this analysis as the base for the future development of a 3D localization technique applicable to living cells. (More)

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Paper citation in several formats:
Sokoll, S.; Tönnies, K. and Heine, M. (2011). TOWARDS FAST 3D NANOPARTICLE LOCALIZATION FOR STUDYING MOLECULAR DYNAMICS IN LIVING CELLS.In Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011) ISBN 978-989-8425-36-2, pages 210-215. DOI: 10.5220/0003166502100215

@conference{bioinformatics11,
author={Stefan Sokoll. and Klaus Tönnies. and Martin Heine.},
title={TOWARDS FAST 3D NANOPARTICLE LOCALIZATION FOR STUDYING MOLECULAR DYNAMICS IN LIVING CELLS},
booktitle={Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011)},
year={2011},
pages={210-215},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003166502100215},
isbn={978-989-8425-36-2},
}

TY - CONF

JO - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011)
TI - TOWARDS FAST 3D NANOPARTICLE LOCALIZATION FOR STUDYING MOLECULAR DYNAMICS IN LIVING CELLS
SN - 978-989-8425-36-2
AU - Sokoll, S.
AU - Tönnies, K.
AU - Heine, M.
PY - 2011
SP - 210
EP - 215
DO - 10.5220/0003166502100215

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