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Authors: Emilee E. Colón-Lorenzo 1 ; Adelfa E. Serrano 1 ; Hugh B. Nicholas Jr 2 ; Troy Wymore 2 ; Alexander J. Ropelewski 2 and Ricardo González-Méndez 1

Affiliations: 1 University of Puerto Rico School of Medicine, Puerto Rico ; 2 Pittsburgh Supercomputing Center, United States

ISBN: 978-989-674-019-1

Keyword(s): Malaria, Plasmodium, Glutathione S-transferase, Bioinformatics analysis, Structural alignment, GST classification, Structural modeling.

Related Ontology Subjects/Areas/Topics: Bioinformatics ; Biomedical Engineering ; Sequence Analysis ; Structural Bioinformatics

Abstract: Malaria is a global health problem caused by Plasmodium parasites. Glutathione S-transferase (GST) is involved in the conjugation of glutathione to drugs and toxic compounds. It is postulated that GST plays an important role in the development of drug resistance. The three-dimensional (3D) structure of Plasmodium falciparum GST (PfGST) has been solved and previous work indicates that the PfGST cannot be assigned to any of the known GST classes. We performed sequence analyses, structural modeling and alignment of GSTs from Plasmodium to known structures of the GST from other organisms to classify PfGST into a GST family. Sequence alignments using ClustalW, motif analysis using MEME, and phylogenetic analysis using MEGA4, of Plasmodium GSTs and 38 other GST sequences were done. The alignments and motifs show a close relationship to the alpha and sigma class of GSTs. The phylogenetic analysis places the Plasmodium GSTs in the sigma class. A comparison of PfGST with known structures of GS Ts reveals high structural similarity to the sigma class GST, in particular within the H-site and C-terminus of the protein. These findings allow PfGST to be classified into the sigma class GSTs. These data may open new avenues for the development of novel antimalarials. (More)

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Paper citation in several formats:
E. Colón-Lorenzo E.; E. Serrano A.; B. Nicholas Jr H.; Wymore T.; J. Ropelewski A.; González-Méndez R. and (2010). THE PLASMODIUM GLUTATHIONE S-TRANSFERASE - Bioinformatics Characterization and Classification into the Sigma Class.In Proceedings of the First International Conference on Bioinformatics - Volume 1: BIOINFORMATICS, (BIOSTEC 2010) ISBN 978-989-674-019-1, pages 173-180. DOI: 10.5220/0002747401730180

@conference{bioinformatics10,
author={Emilee {E. Colón{-}Lorenzo} and Adelfa {E. Serrano} and Hugh {B. Nicholas Jr} and Troy Wymore and Alexander {J. Ropelewski} and Ricardo González{-}Méndez},
title={THE PLASMODIUM GLUTATHIONE S-TRANSFERASE - Bioinformatics Characterization and Classification into the Sigma Class},
booktitle={Proceedings of the First International Conference on Bioinformatics - Volume 1: BIOINFORMATICS, (BIOSTEC 2010)},
year={2010},
pages={173-180},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0002747401730180},
isbn={978-989-674-019-1},
}

TY - CONF

JO - Proceedings of the First International Conference on Bioinformatics - Volume 1: BIOINFORMATICS, (BIOSTEC 2010)
TI - THE PLASMODIUM GLUTATHIONE S-TRANSFERASE - Bioinformatics Characterization and Classification into the Sigma Class
SN - 978-989-674-019-1
AU - E. Colón-Lorenzo, E.
AU - E. Serrano, A.
AU - B. Nicholas Jr, H.
AU - Wymore, T.
AU - J. Ropelewski, A.
AU - González-Méndez, R.
PY - 2010
SP - 173
EP - 180
DO - 10.5220/0002747401730180

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