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Authors: Masataka Shiozawa and Shigeyasu Uno

Affiliation: Graduate School of Science and Engineering, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, Shiga, 525-8577, Japan

Keyword(s): Electrochemical Impedance, Simulation, Single Cell Analysis, Microelectrode.

Abstract: Electrochemical impedance spectroscopy (EIS) is one of the ways to monitor living cells in terms of morphology and cell-substrate adhesion. In conventional cell analysis, averaged values from many cells on a large area electrode are acquired. Meanwhile, single cell characteristics are obtained by using a microelectrode which is smaller than single cell size. In this study, we investigated the impact of electrode size on single cell EIS analysis by using a computer simulation. The electrode smaller than the cell size would enable independent measurement of the cytoplasm electrical conductivity and the cell-substrate gap resistance. In addition, changes in morphology such as cell height and radius should be monitored at the same time in different frequency range. Thus, our simulation indicates that EIS measurement with microscale electrode may be used in monitoring various physiological changes in cells such as immune response.

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Paper citation in several formats:
Shiozawa, M. and Uno, S. (2021). Electrochemical Impedance Simulation for Single Cell Analysis using a Microelectrode. In Proceedings of the 14th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2021) - BIODEVICES; ISBN 978-989-758-490-9; ISSN 2184-4305, SciTePress, pages 114-120. DOI: 10.5220/0010266400002865

@conference{biodevices21,
author={Masataka Shiozawa. and Shigeyasu Uno.},
title={Electrochemical Impedance Simulation for Single Cell Analysis using a Microelectrode},
booktitle={Proceedings of the 14th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2021) - BIODEVICES},
year={2021},
pages={114-120},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010266400002865},
isbn={978-989-758-490-9},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the 14th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2021) - BIODEVICES
TI - Electrochemical Impedance Simulation for Single Cell Analysis using a Microelectrode
SN - 978-989-758-490-9
IS - 2184-4305
AU - Shiozawa, M.
AU - Uno, S.
PY - 2021
SP - 114
EP - 120
DO - 10.5220/0010266400002865
PB - SciTePress

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