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Authors: Peter D. Dowd 1 ; Jan Karolin 2 ; Carol Trager-Cowan 2 ; David J. S. Birch 2 and William H. Stimson 3

Affiliations: 1 University of Strathclyde, United Kingdom ; 2 Centre for Molecular Nanometrology, University of Strathclyde and SUPA, United Kingdom ; 3 Strathclyde Institute of Pharmacy and Biomedical Science and University of Strathclyde, United Kingdom

Keyword(s): Homogeneous immunoassay, Time-resolved fluorescence depolarisation, Gonadotropin-releasing hormone.

Related Ontology Subjects/Areas/Topics: Biomedical Engineering ; Biomedical Instruments and Devices ; Biomedical Sensors ; Spectroscopic Applications

Abstract: A new method for detecting gonadotropin-releasing hormone type 1 (GnRH-1) is proposed. The method is based on a fluorescence polarisation immunoassay where the GnRH-1 vies for the binding site on the anti-GnRH (antibody 7B10.1D10) with a labelled peptide consisting of a fragment of GnRH labelled with fluorescein. Time-resolved anisotropy decays indicate increased correlation times for the labelled fragment in the presence of 7B10.1D10 compared with the correlation times for the labelled peptide fragment alone. When GnRH-1 is added to the solution of the labelled peptide in the presence of 7B10.1D10 it has the effect of reducing the correlation times. The results demonstrate the possibility of introducing the labelled fragment as a competitor to the antigen in the fluorescence polarisation immunoassay for GnRH-1 and, more generally, in the case where there is a disadvantage in labelling the antigen, such as GnRH-1, with a fluorescent probe.

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Paper citation in several formats:
Dowd, P.; Karolin, J.; Trager-Cowan, C.; S. Birch, D. and Stimson, W. (2011). TOWARDS A NEW HOMOGENEOUS IMMUNOASSAY FOR GONADOTROPIN-RELEASING HORMONE BASED ON TIME-RESOLVED FLUORESCENCE ANISOTROPY. In Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2011) - BIODEVICES; ISBN 978-989-8425-37-9; ISSN 2184-4305, SciTePress, pages 184-188. DOI: 10.5220/0003152001840188

@conference{biodevices11,
author={Peter D. Dowd. and Jan Karolin. and Carol Trager{-}Cowan. and David J. {S. Birch}. and William H. Stimson.},
title={TOWARDS A NEW HOMOGENEOUS IMMUNOASSAY FOR GONADOTROPIN-RELEASING HORMONE BASED ON TIME-RESOLVED FLUORESCENCE ANISOTROPY},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2011) - BIODEVICES},
year={2011},
pages={184-188},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003152001840188},
isbn={978-989-8425-37-9},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2011) - BIODEVICES
TI - TOWARDS A NEW HOMOGENEOUS IMMUNOASSAY FOR GONADOTROPIN-RELEASING HORMONE BASED ON TIME-RESOLVED FLUORESCENCE ANISOTROPY
SN - 978-989-8425-37-9
IS - 2184-4305
AU - Dowd, P.
AU - Karolin, J.
AU - Trager-Cowan, C.
AU - S. Birch, D.
AU - Stimson, W.
PY - 2011
SP - 184
EP - 188
DO - 10.5220/0003152001840188
PB - SciTePress

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