Authors:
Bin Wang
1
;
Baidong Zhang
2
and
Yibing Zhou
1
Affiliations:
1
Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University and Dalian, China
;
2
Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences and Qingdao, China
Keyword(s):
Polychaete, bioremediation, digestive tract, Pseudoalteromonas Haloplanktis
Abstract:
The application of bioremediation approaches employing hydrocarbon-utilizing microorganisms to remove petroleum hydrocarbons from oil spills is an area of research that has gained extensive attention and has been widely investigated. In the present study, attempts have been made to isolate and characterize hydrocarbon-utilizing microorganisms immobilized in the digestive tract of Perinereis aibuhitensis. Isolate SC11-3, a potent petroleum-degrading organism, from Perinereis aibuhitensis gut samples was identified as Pseudoalteromonas sp. A detailed morphological, biochemical, and 16S rDNA sequence analysis revealed that it was closely related to Pseudoalteromonas haloplanktis. The isolate SC11-3 was capable of consuming about 40% diesel within 15 days from the medium containing 1 ml L-1 of oil. Furthermore, it was observed that the degrading efficiency of the isolate SC11-3 was significantly enhanced up to approximately 90% when the medium was supplemented with 4 g L-1 of glucose, in
dicating the possible occurrence of co-metabolism during the process of petroleum degradation by the bacterium. Our study reported an isolate of petroleum-degrading bacterium and its potential co-metabolism mechanism in oil degradation processes, which will provide new insight into in situ bioremediation of multi-biological systems.
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