Authors:
Hongbin Guo
;
Jianzhi Ye
;
Yuanjing Liu
;
Ling Lin
;
Chunliang Yang
and
Qi Li
Affiliation:
Agricultural Products Processing Institute, CATAS, Zhanjiang 524001 and China, China
Keyword(s):
Phorate, UPLC-MS/MS, Egg, Determination
Abstract:
In this study, we present a newly developed fast and sensitive method for the quantification of phorate and its metabolite phorate sulfone residues in the egg, using solid phase extraction and UPLC-MS/MS analysis.The eggs sample were extracted with acetonitrile under neutral conditions in this paper , cleaned up by the glass funnel plugged with cotton and salts equipped with anhydrous sodium sulfate and neutral alumina, and determinatied by waters high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). There are good linear correlations between the peak area and the corresponding concentration of phorate and its metabolite phorate sulfone in the range of 2.00-100.00 ng/mL and the linear coefficient of the three pesticides is above 0.9995. The recovery rate of phorate and its metabolite phorate sulfone ranged from 78.6% to 95.6%, and with the batch relative deviation ranged from 0.3% to 5.6%. The lower limit detection (LOD) of the phorate sulfone, phorate and pho
rate sulfoxide were 0.0005 mg/kg , 0.0005mg/kg and 0.0005 mg/kg, respectively. The phorate, phorate sulfone and phorate sulfoxide's quantitative(LOQ)were 0.0015 mg/kg, 0.0015 mg/kg and 0.0015 mg/kg, respectively. The results show that UPLC-MS/MS in conjunction with phorate and its metabolite phorate sulfone is a powerful tool for screening the inhibitory effects of eggs and can thus be used for safety control in food processing procedures.
(More)