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Authors: P. Krämer 1 ; F. Boto 1 ; D. Wald 1 ; F. Bessy 1 ; C. Paloc 1 ; C. Callol 2 ; A. Letamendia 2 ; I. Ibarbia 2 ; O. Holgado 2 and J. M. Virto 2

Affiliations: 1 Vicomtech, Spain ; 2 Biobide, Spain

Keyword(s): Segmentation, Fluorescent microscopy images, Embryonic heart.

Related Ontology Subjects/Areas/Topics: Applications and Services ; Biomedical Engineering ; Biomedical Signal Processing ; Computer Vision, Visualization and Computer Graphics ; Medical Image Detection, Acquisition, Analysis and Processing

Abstract: Embryos of animal models are becoming widely used to study cardiac development and genetics. However, the analysis of the embryonic heart is still mostly done manually. This is a very laborious and expensive task as each embryo has to be inspected visually by a biologist. We therefore propose to automatically segment the embryonic heart from high-speed fluorescence microscopy image sequences, allowing morphological and functional quantitative features of cardiac activity to be extracted. Several methods are presented and compared within a large range of images, varying in quality, acquisition parameters, and embryos position. Although manual control and visual assessment would still be necessary, the best of our methods has the potential to drastically reduce biologist workload by automating manual segmentation.

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Paper citation in several formats:
Krämer, P.; Boto, F.; Wald, D.; Bessy, F.; Paloc, C.; Callol, C.; Letamendia, A.; Ibarbia, I.; Holgado, O. and Virto, J. (2010). AUTOMATIC SEGMENTATION OF EMBRYONIC HEART IN TIME-LAPSE FLUORESCENCE MICROSCOPY IMAGE SEQUENCES. In Proceedings of the Third International Conference on Bio-inspired Systems and Signal Processing (BIOSTEC 2010) - BIOSIGNALS; ISBN 978-989-674-018-4; ISSN 2184-4305, SciTePress, pages 121-126. DOI: 10.5220/0002342501210126

@conference{biosignals10,
author={P. Krämer. and F. Boto. and D. Wald. and F. Bessy. and C. Paloc. and C. Callol. and A. Letamendia. and I. Ibarbia. and O. Holgado. and J. M. Virto.},
title={AUTOMATIC SEGMENTATION OF EMBRYONIC HEART IN TIME-LAPSE FLUORESCENCE MICROSCOPY IMAGE SEQUENCES},
booktitle={Proceedings of the Third International Conference on Bio-inspired Systems and Signal Processing (BIOSTEC 2010) - BIOSIGNALS},
year={2010},
pages={121-126},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0002342501210126},
isbn={978-989-674-018-4},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the Third International Conference on Bio-inspired Systems and Signal Processing (BIOSTEC 2010) - BIOSIGNALS
TI - AUTOMATIC SEGMENTATION OF EMBRYONIC HEART IN TIME-LAPSE FLUORESCENCE MICROSCOPY IMAGE SEQUENCES
SN - 978-989-674-018-4
IS - 2184-4305
AU - Krämer, P.
AU - Boto, F.
AU - Wald, D.
AU - Bessy, F.
AU - Paloc, C.
AU - Callol, C.
AU - Letamendia, A.
AU - Ibarbia, I.
AU - Holgado, O.
AU - Virto, J.
PY - 2010
SP - 121
EP - 126
DO - 10.5220/0002342501210126
PB - SciTePress