Authors:
Nurul Amalia
1
;
Agung Dwi Wahyu Widodo
2
and
Willy Sandhika
3
Affiliations:
1
Departement of Immunology Postgraduate School Airlangga University Surabaya and East Java, Indonesia
;
2
Departement of Microbiology Clinic, Dr. Soetomo Hospital Surabaya and East Java, Indonesia
;
3
Departement of Anatomy Pathology, Faculty of Medicine, Airlangga University Surabaya and Jawa Timur, Indonesia
Keyword(s):
Apoptosis, Caspase-3, Streptococcus pyogenes, Acinetobacter baumannii.
Abstract:
Streptococcus pyogenes as exotoxin-producing bacteria are the most common cause of pharyngitis infection. Acinetobacter baumannii as endotoxin-producing bacteria are found in nosocomial infections. The infections that has already spread to all organs with decreased immune system undergo apoptosis in liver and spleen. Caspase-3 is a death protease most commonly activated by apoptotic mediator. The higher the caspase-3 expression, the higher the severity of the disease, which can cause the organ to experience dysfunction or failure. This study was aimed to observe the expression of caspase-3 in the liver and spleen of Rattus norvegicus, infected by Streptococcus pyogenes and Acinetobacter baumannii. This is a true experimental with a post-test only control-group design. The healthy rats were randomly selected and injected with 1ml (PZ, suspension of bacteri A. baumannii and S. pyogenes) in the peritoneum in quadrant 3 for each group of experimental animals and observed for 24 hours. Af
ter 24 hours, surgery took place for the removal of the liver and spleen. Organ tissues where fixed into a formalin buffer and tissue was prepared for IHC caspase-3.The results showed that the mortality rate of Rattus norvegicus infected by A. baumannii was higher than those infected by S. pyogenes. Caspase-3 expression in the liver of A. baumannii group was 48, S. pyogenes was 22.5, and the control group was 9.5. The mean value of the caspase-3 index in the spleen of the A. baumannii group was 28.5, S. pyogenes was 17, and the control group was 4.
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