A Study on Enhanced Fluorescence Signal-to-noise by using the Stray Light Shutter for Quantitative PCR Chip

Liang-Chieh Chao, Chun-Han Chou, Hsin-Yi Tsai, Kuo-Cheng Huang, Dar-Bin Shieh

Abstract

Quantitative polymerase chain reaction (qPCR) is the most important inspection technique for virus, especially for coronavirus disease, in this year. The qPCR chip and device were planned to develop because of the characteristics of fast inspection time, high accuracy and small system volume. Therein, the fluorescence intensity was the important signal in qPCR device, which represented the positive or negative reaction after the DNA was amplified and bound on the fluorescence dye, but the fluorescence signal was easily to be affected by the excitation and scattered light. The mini spectrometer was employed to receive the fluorescence intensity in PCR chip in this study, and the optical simulation was progressed, and the stray light shutter (SLS) was added to improve the signal-to-noise ratio (SNR) of fluorescence. The analysis results showed that the SNR of fluorescence can be enhanced from 3.14 to 16.78 by using the SLS with shape of extend component aperture, which the protruding structure was at the direction away from center. The results from this manuscript can provide the important reference information to the developer of qPCR chip, whom can obtain the high SNR fluorescence signal in qPCR inspection process for disease.

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Paper Citation


in Harvard Style

Chao L., Chou C., Tsai H., Huang K. and Shieh D. (2021). A Study on Enhanced Fluorescence Signal-to-noise by using the Stray Light Shutter for Quantitative PCR Chip.In Proceedings of the 9th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS, ISBN 978-989-758-492-3, pages 54-60. DOI: 10.5220/0010362500540060


in Bibtex Style

@conference{photoptics21,
author={Liang-Chieh Chao and Chun-Han Chou and Hsin-Yi Tsai and Kuo-Cheng Huang and Dar-Bin Shieh},
title={A Study on Enhanced Fluorescence Signal-to-noise by using the Stray Light Shutter for Quantitative PCR Chip},
booktitle={Proceedings of the 9th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS,},
year={2021},
pages={54-60},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010362500540060},
isbn={978-989-758-492-3},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 9th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS,
TI - A Study on Enhanced Fluorescence Signal-to-noise by using the Stray Light Shutter for Quantitative PCR Chip
SN - 978-989-758-492-3
AU - Chao L.
AU - Chou C.
AU - Tsai H.
AU - Huang K.
AU - Shieh D.
PY - 2021
SP - 54
EP - 60
DO - 10.5220/0010362500540060