Effects of Electrical Fields on Neuroblastoma (N2A) Cell Differentiation: Preliminary Results

Daniel Fernández, Pablo García, María Martín, Paula Daza, Juan Serrano-Viseas, Gloria Huertas, Alberto Yúfera

Abstract

This work describes Electrical Stimulations (ES) assays on stem cells. The neuroblastoma (N2A) cell linage was submitted to several electrical fields to enable and enhance its differentiation toward neurons. Both Direct Current (DC) and Alternated Current (AC) time dependent electric field protocols were applied to N2A cell culture under differentiation conditions, obtaining different responses. Control and electrically excited samples’ number of differentiated cells and neurite lengths were measure after differentiation. Results showed that DC fields have a strong influence on N2A differentiation since the percentage of differentiated cells and the neurites lengths were the highest. In addition, a significant alignment of neurites measured with the applied electrical field has been detected, which demonstrates the high sensitivity of differentiation processes to electrical field polarity.

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Paper Citation


in Harvard Style

Fernández D., García P., Martín M., Daza P., Serrano-Viseas J., Huertas G. and Yúfera A. (2021). Effects of Electrical Fields on Neuroblastoma (N2A) Cell Differentiation: Preliminary Results.In Proceedings of the 14th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, ISBN 978-989-758-490-9, pages 152-159. DOI: 10.5220/0010320101520159


in Bibtex Style

@conference{biodevices21,
author={Daniel Fernández and Pablo García and María Martín and Paula Daza and Juan Serrano-Viseas and Gloria Huertas and Alberto Yúfera},
title={Effects of Electrical Fields on Neuroblastoma (N2A) Cell Differentiation: Preliminary Results},
booktitle={Proceedings of the 14th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES,},
year={2021},
pages={152-159},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010320101520159},
isbn={978-989-758-490-9},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 14th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES,
TI - Effects of Electrical Fields on Neuroblastoma (N2A) Cell Differentiation: Preliminary Results
SN - 978-989-758-490-9
AU - Fernández D.
AU - García P.
AU - Martín M.
AU - Daza P.
AU - Serrano-Viseas J.
AU - Huertas G.
AU - Yúfera A.
PY - 2021
SP - 152
EP - 159
DO - 10.5220/0010320101520159