Quantification of Lactic Acid as Secondary Metabolite of Lactic Acid Bacteria Isolated from Milk and Its Derivatived Products

Fifi Afiati, Fitri Setiyoningrum, Gunawan Priadi, Vania Qyasaty

Abstract

Lactic acid is a widely used secondary metabolite product. Titrated Total Acid (TTA) analysis is one of method to learn quantification process of lactic acid as a secondary metabolite of Lactic Acid Bacteria (LAB). The TTA test was carried out on 158 isolates from 11 samples with 2 replications using culture supernatant. The test was started with preparing growth media then rejuvenating the culture. NaOH 0.1 N, oxalic acid 0.1 N, and PP 1% indicator were prepared as reagents for analysis. As the result, the smallest TTA value is shown in the 1-Sa-L sample with a TTA value of 0.41% and the largest TTA value is shown in the KGL-5 sample with a TTA value of 1.56%.

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Paper Citation


in Harvard Style

Afiati F., Setiyoningrum F., Priadi G. and Qyasaty V. (2020). Quantification of Lactic Acid as Secondary Metabolite of Lactic Acid Bacteria Isolated from Milk and Its Derivatived Products. In Proceedings of the 6th Food Ingredient Asia Conference - Volume 1: 6th FiAC, ISBN 978-989-758-540-1, pages 132-136. DOI: 10.5220/0010546700003108


in Bibtex Style

@conference{6th fiac20,
author={Fifi Afiati and Fitri Setiyoningrum and Gunawan Priadi and Vania Qyasaty},
title={Quantification of Lactic Acid as Secondary Metabolite of Lactic Acid Bacteria Isolated from Milk and Its Derivatived Products},
booktitle={Proceedings of the 6th Food Ingredient Asia Conference - Volume 1: 6th FiAC,},
year={2020},
pages={132-136},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010546700003108},
isbn={978-989-758-540-1},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 6th Food Ingredient Asia Conference - Volume 1: 6th FiAC,
TI - Quantification of Lactic Acid as Secondary Metabolite of Lactic Acid Bacteria Isolated from Milk and Its Derivatived Products
SN - 978-989-758-540-1
AU - Afiati F.
AU - Setiyoningrum F.
AU - Priadi G.
AU - Qyasaty V.
PY - 2020
SP - 132
EP - 136
DO - 10.5220/0010546700003108