IL-6 and IL-8 Suppression by Bacteria-adhered Mesenchymal Stem Cells Co-cultured with PBMCs under TNF-α Exposure

Agung Putra, Agung Putra, Agung Putra, Iffan Alif, Mohammad Ariq Nazar, Ardi Prasetio, Risky Chandra Satria Irawan, Dina Amalina, Dina Amalina, Endah Permata Sari, Azizah Retno Kustiyah, Iqbal Pahlevi Adeputra Nasution

Abstract

The potential of mesenchymal stem cells (MSCs) in controlling bacterial infections is an evolving field to investigate. In terms of response to inflammatory cytokines, MSCs can polarize into MSCs type-1 and MSCs type-2 to reach the homeostasis process, including regulating IL-8 and IL-6. MSCs are also exhibit antimicrobial properties and regulate immune responses. This study was designed to explore the ability of MSCs to control the inflammation produced by Staphylococcus aureus-contaminated PBMC with TNF-α stimulation by analyzing IL-6 and IL-8 levels. We used a post-test group design with 2 study groups, consist of vehicle control (Veh) and a treatment (1:20 comparison of MSCs: PBMCs) in triplicate supplemented with S. aureus under 10 ng/mL TNF-α recombinant. The medium supernatant was collected after 0, 4, 8, and 12, the IL-6 and IL-8 were measured using ELISA assay. This study showed a significant increase in IL-6 and IL-8 at the first hour’s incubation. Interestingly, the IL-6 and IL-8 levels were significantly decreased after 12 and 8 to 12 hours of incubation, respectively. Based on our study, we conclude that MSCs may regulate the IL-6 and IL-8 production on bacteria-contaminated PBMC with inflammation in early incubation to late incubation.

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Paper Citation


in Harvard Style

Putra A., Alif I., Nazar M., Prasetio A., Satria Irawan R., Amalina D., Sari E., Kustiyah A. and Adeputra Nasution I. (2020). IL-6 and IL-8 Suppression by Bacteria-adhered Mesenchymal Stem Cells Co-cultured with PBMCs under TNF-α Exposure. In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 311-317. DOI: 10.5220/0010491903110317


in Bibtex Style

@conference{jimc20,
author={Agung Putra and Iffan Alif and Mohammad Ariq Nazar and Ardi Prasetio and Risky Chandra Satria Irawan and Dina Amalina and Endah Permata Sari and Azizah Retno Kustiyah and Iqbal Pahlevi Adeputra Nasution},
title={IL-6 and IL-8 Suppression by Bacteria-adhered Mesenchymal Stem Cells Co-cultured with PBMCs under TNF-α Exposure},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={311-317},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010491903110317},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - IL-6 and IL-8 Suppression by Bacteria-adhered Mesenchymal Stem Cells Co-cultured with PBMCs under TNF-α Exposure
SN - 978-989-758-499-2
AU - Putra A.
AU - Alif I.
AU - Nazar M.
AU - Prasetio A.
AU - Satria Irawan R.
AU - Amalina D.
AU - Sari E.
AU - Kustiyah A.
AU - Adeputra Nasution I.
PY - 2020
SP - 311
EP - 317
DO - 10.5220/0010491903110317