Efficacy of Topical Binahong Leaf Ethanolic Extract Administration on Serum TGF-β1 in Wistar Rats with Staphylococcus aureus-infected Wounds

Rizki Andini Nawawi, Muhammad Totong Kamaluddin, Theodorus

Abstract

Binahong (Anredera cordifolia (Ten.) Steenis) is a medicinal plant traditionally used as a topical wound treatment. Saponin content in Binahong leaf extract has been thought to increase TGF-β secretion, which plays an important role in accelerating wound healing. This study’s aim was to assess the efficacy of topical Binahong leaf ethanolic extract administration on serum TGF-β1 in infected wounds. An experimental study, in vivo, was conducted in the Biotechnology Laboratory and Animal House, Faculty of Medicine, Universitas Sriwijaya, Palembang, from July to September 2020. Thirty male Wistar rats aged 10-12 weeks with excisional wounds were infected with Staphylococcus aureus ATCC 25923. The rats were divided into five groups and received three concentrations of Binahong leaf extracts (2.5%, 5%, and 10%), salve base, and povidone iodine 10% topically twice daily for 14 days. Serum was obtained before treatment and after day 14 of treatment. Wound area was also recorded. After 14 days of topical administration of Binahong leaf extract on rats with Staphylococcus aureus-infected wounds, a decrease in wound size was most significantly observed in rats receiving 10% Binahong leaf extract (p = 0.02), but no significant serum TGF-β1 increase was observed in all treatment groups.

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Paper Citation


in Harvard Style

Nawawi R., Kamaluddin M. and Theodorus. (2020). Efficacy of Topical Binahong Leaf Ethanolic Extract Administration on Serum TGF-β1 in Wistar Rats with Staphylococcus aureus-infected Wounds.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 267-272. DOI: 10.5220/0010491202670272


in Bibtex Style

@conference{jimc20,
author={Rizki Andini Nawawi and Muhammad Totong Kamaluddin and Theodorus},
title={Efficacy of Topical Binahong Leaf Ethanolic Extract Administration on Serum TGF-β1 in Wistar Rats with Staphylococcus aureus-infected Wounds},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={267-272},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010491202670272},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Efficacy of Topical Binahong Leaf Ethanolic Extract Administration on Serum TGF-β1 in Wistar Rats with Staphylococcus aureus-infected Wounds
SN - 978-989-758-499-2
AU - Nawawi R.
AU - Kamaluddin M.
AU - Theodorus.
PY - 2020
SP - 267
EP - 272
DO - 10.5220/0010491202670272