The Effect of Caffeine towards Zebrafish (Danio rerio) Juvenile Working Memory Exposed by Unpredictable Chronic Stress (UCS)

Alya Ayu Tazkia, Zainuri Sabta Nugraha

Abstract

The changes in brain structure are caused by dependent and independent factors. Early Life Stress (ELS) is one of the dependent factors that affect the brain’s structure and volume. ELS exposure increases synaptic pruning in the neuron in which it disturbs the cognitive function, memory loss, emotion, and risk-taking. Researchers strive to perceive the effect of caffeine in coffee, which can increase memory. Caffeine in coffee has an active compound which increases memory. This study aimed to identify the effect of caffeine in coffee to memory with exposure to stress. The method used in this research was experimental using a post-test with controlled group design. The group were divided into four groups comprising of Negative Control (K1), Positive Control (K2), and group in which exposed by caffeine using different doses (P1 and P2). The exposed group used 20mg/dL and 50mg/dL doses. Hence, the exposure was accorded by the protocol of toxicity for three days. After three days of exposure, group K2, P and P2 were given Unpredictable Chronic Stress (UCS) intervention for seven days. All of the groups were tested using T-maze for three days to see the Zebrafish’s memory. Data analysis were collected from Zebrafish time and selection of colour on every side of T-maze. This study shows that there are differences in each group according to time, the average group of K1 (27,11 s), K2 (41,01 s), P1 (25,62 s), P2 (49,22 s). The fastest time was shown in P1. Meanwhile, the slowest time was shown in P2. The test using Shapiro-Wilk showed (P=0,000) p>0,005 in which the data were not well distributed. The data normality using Kruskal-Wallis showed (P=0,000) p<0,005. Hence, H1 can be accepted. Post-hoc test for every group according to time showed group P1-K1, P1-P2, K1-K2, K1-P2 (p<0,005), whereas K1-P1 and K2-P2 (0,063) (p>0,005). Otherwise, compared group according to colour using Chi-Square showed a significant difference (p<0,005). As a conclusion, study report the exposure of caffeine and intervention of UCS in Zebrafish affect memory in the T-maze test.

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Paper Citation


in Harvard Style

Tazkia A. and Nugraha Z. (2020). The Effect of Caffeine towards Zebrafish (Danio rerio) Juvenile Working Memory Exposed by Unpredictable Chronic Stress (UCS).In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 208-213. DOI: 10.5220/0010490202080213


in Bibtex Style

@conference{jimc20,
author={Alya Ayu Tazkia and Zainuri Sabta Nugraha},
title={The Effect of Caffeine towards Zebrafish (Danio rerio) Juvenile Working Memory Exposed by Unpredictable Chronic Stress (UCS)},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={208-213},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010490202080213},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - The Effect of Caffeine towards Zebrafish (Danio rerio) Juvenile Working Memory Exposed by Unpredictable Chronic Stress (UCS)
SN - 978-989-758-499-2
AU - Tazkia A.
AU - Nugraha Z.
PY - 2020
SP - 208
EP - 213
DO - 10.5220/0010490202080213