The Effects of Aqueous Extract of Jamaican Cherry (Muntingia calabura) on D-galactose-induced Liver Damage in BALB/c Mice (Mus musculus)

Cempaka Jaga Pramudita, Dwi Nur Ahsani, Ika Fidianingsih, Evy Sulistyoningrum

Abstract

D-Galactose induces oxidative stress that will damage the hepatocytes. The aqueous extract of Muntingia calabura leaves (AQMC) is known to have a potential antioxidant to prevent hepatocyte damage caused by oxidative stress. This study aimed to determine the effects of AQMC leaf extract on liver damage induced by D-Galactose in BALB/c Mus musculus. This was experimental research with a completely-randomized design involving 20 hepatic samples (paraffin blocks and HE staining) from 5 groups. The groups included K1 (healthy), K2 (D-galactose-induced), K3 (D-galactose + AQMC 35 mg/kgBW), K4 (D-galactose + AQMC 75mg/kgBW), and K5 (D-galactose + vitamin C 28mg/KgBW). D-galactose was administered for 6 weeks prior to the therapy (vitamin C or AQMC given for 4 weeks). Liver damage was observed in all fields of view and described comprehensively. The degree of hepatocyte damage was calculated using the Manja Roenigk scoring and analyzed using One-way ANOVA with post-hoc Tukey’s HSD test (CI = 95%, α = 0.05). AQMC leaf extract could reduce D-galactose-induced liver damage in BALB/c Mus musculus. The hepatocyte damage in the groups given AQMC therapy was less than that in the D-galactose negative control group (K1 = 73.5 + 2.39, K2 = 92.00 + 5.24, K3 = 69.00 + 2.79, K4 = 76.25 + 4.42, K5 = 77.25 + 6.48; p = 0.029). AQMC at a dose of 35mg/KgBW showed more effective therapeutic potential against D-galactose (K3 = 0.003, K4 = 0.027; post-hoc toward K2). AQMC administration could reduce liver and hepatocyte damage of BALB/c Mus musculus induced by D-Galactose at a potential dose of 35 mg.

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Paper Citation


in Harvard Style

Pramudita C., Ahsani D., Fidianingsih I. and Sulistyoningrum E. (2020). The Effects of Aqueous Extract of Jamaican Cherry (Muntingia calabura) on D-galactose-induced Liver Damage in BALB/c Mice (Mus musculus).In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 202-207. DOI: 10.5220/0010490102020207


in Bibtex Style

@conference{jimc20,
author={Cempaka Jaga Pramudita and Dwi Nur Ahsani and Ika Fidianingsih and Evy Sulistyoningrum},
title={The Effects of Aqueous Extract of Jamaican Cherry (Muntingia calabura) on D-galactose-induced Liver Damage in BALB/c Mice (Mus musculus)},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={202-207},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010490102020207},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - The Effects of Aqueous Extract of Jamaican Cherry (Muntingia calabura) on D-galactose-induced Liver Damage in BALB/c Mice (Mus musculus)
SN - 978-989-758-499-2
AU - Pramudita C.
AU - Ahsani D.
AU - Fidianingsih I.
AU - Sulistyoningrum E.
PY - 2020
SP - 202
EP - 207
DO - 10.5220/0010490102020207