Different Contribution of Estrogen Receptors, ET-1/ETBR and Superoxide Dismutase and in eNOS Availibility based on Sexual Dimorphism in Early Stage of Kidney Diabetic Rats

Anisa Fatwa, Anisa Fatwa, Anisa Fatwa, Dwi Cahyani Ratna Sari, Wiwit Ananda Wahyu Setyaningsih, Andrew Nobiantoro, Nur Arfian

Abstract

Background Diabetes mellitus is metabolic diseases which is influenced by multifactorial conditions. Many factors may contribute to DM progression, such as vasoconstrictor and vasodilator balance, as underlying factor of endothelial dysfunction. Sex difference in male and female phenotype has not been elucidated in DM progression, especially in eNOS availibility which associate with endothelin/endothelin receptors and estrogen signaling. This research aims to elucidate the expression of ERα and Erβ, ppET1 via ETBR as vasodilator properties, then SOD1 and SOD2 as antioxidant in male and female DM rats. Diabetes Mellitus was induced in male and female rats with Streptozotocin 60mg/Kg.BB single injection intraperitonially. Control group was injected with NaCl 0.9%. Rats were terminated at the 1st month. Proteinuria score and histological structure were determined in all groups. Reverse Transcription-PCR was performed to know mRNA expression of eNOS, ETBR, ERα and Erβ, SOD1 also SOD2 from kidney. All groups showed that there were no significant differences in proteinuria score and histological structure related to persistent of eNOS mRNA expression which confirmed by RT-PCR analysis. There were no significant differences between eNOS mRNA expression in DM to control groups in each sex. Furthermore, ERα and ERβ mRNA expression in female were significantly higher than male diabetic rats. Nevertheless, ETBR mRNA expression was significantly higher in male compared to female diabetic rats. Then, female diabetic groups had higher SOD1 and SOD2 mRNA expression compared to male. ERs and SOD upregulation in early diabetic female rats and ETBR upregulation in early diabetic male rats might be associated with persistent eNOS expression in early diabetic condition.

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in Harvard Style

Fatwa A., Ratna Sari D., Wahyu Setyaningsih W., Nobiantoro A. and Arfian N. (2020). Different Contribution of Estrogen Receptors, ET-1/ETBR and Superoxide Dismutase and in eNOS Availibility based on Sexual Dimorphism in Early Stage of Kidney Diabetic Rats.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 175-183. DOI: 10.5220/0010489701750183


in Bibtex Style

@conference{jimc20,
author={Anisa Fatwa and Dwi Cahyani Ratna Sari and Wiwit Ananda Wahyu Setyaningsih and Andrew Nobiantoro and Nur Arfian},
title={Different Contribution of Estrogen Receptors, ET-1/ETBR and Superoxide Dismutase and in eNOS Availibility based on Sexual Dimorphism in Early Stage of Kidney Diabetic Rats},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={175-183},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010489701750183},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Different Contribution of Estrogen Receptors, ET-1/ETBR and Superoxide Dismutase and in eNOS Availibility based on Sexual Dimorphism in Early Stage of Kidney Diabetic Rats
SN - 978-989-758-499-2
AU - Fatwa A.
AU - Ratna Sari D.
AU - Wahyu Setyaningsih W.
AU - Nobiantoro A.
AU - Arfian N.
PY - 2020
SP - 175
EP - 183
DO - 10.5220/0010489701750183