The Activities of Streptomyces W-5A as Antibacterial and Antibiofilm towards Methicillin-resistant Staphylococcus aureus 2983

Annisa Permata Dinda, Ari Asnani, Dwi Utami Anjarwati

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) causes nosocomial infection worldwide. MRSA can defend itself by forming a biofilm layer, thereby increasing the virulence factor. WHO categorizes MRSA as high risk on the Priority Pathogen List for searching for new antibiotics. Recently, we have reported the potency of Streptomyces W-5A as anti-MRSA based on qualitative screening. Thus, this research aimed to analyze the potency of Streptomyces W-5A as antibacterial and antibiofilm towards MRSA 2983. Cultivation of Streptomyces W-5A used Starch Casein Nitrate (SCN) agar, and anti-MRSA extract production used SCN broth. Samples were taken at incubation times of 0, 3, 6, 9, 12, and 15 days. Each sample was tested for antibacterial activity with the Kirby-Bauer method, whereas inhibition of biofilm formation and biofilm degradation with microtiter method. The results showed that the optimum antibacterial activity was achieved after nine days of incubation with an inhibition zone of 9.589 ± 0.521. The optimum % inhibition of biofilm formation was 77.806 ± 13.595% after 12 days of incubation. The optimum % degradation of biofilm was 80.465 ± 7.586% after nine days of incubation. These findings suggest that Streptomyces W-5A has the potency to produce antibacterial and antibiofilm compounds against MRSA 2983.

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Paper Citation


in Harvard Style

Dinda A., Asnani A. and Anjarwati D. (2020). The Activities of Streptomyces W-5A as Antibacterial and Antibiofilm towards Methicillin-resistant Staphylococcus aureus 2983.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 109-115. DOI: 10.5220/0010488601090115


in Bibtex Style

@conference{jimc20,
author={Annisa Permata Dinda and Ari Asnani and Dwi Utami Anjarwati},
title={The Activities of Streptomyces W-5A as Antibacterial and Antibiofilm towards Methicillin-resistant Staphylococcus aureus 2983},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={109-115},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010488601090115},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - The Activities of Streptomyces W-5A as Antibacterial and Antibiofilm towards Methicillin-resistant Staphylococcus aureus 2983
SN - 978-989-758-499-2
AU - Dinda A.
AU - Asnani A.
AU - Anjarwati D.
PY - 2020
SP - 109
EP - 115
DO - 10.5220/0010488601090115