In Vitro Study of Reduction of Oral Enterococcus faecalis Biofilm on Application of Combination of Chrysomya megacephala Maggot Extract and Sodium Hypochlorite

Rizka Hidayati, Ari Asnani, Muhamad Salman Fareza, Dwi Utami Anjarwati

Abstract

The tooth's infected root canal relates to the bacteria invasion, such as Enterococcus faecalis. The eradication of the bacteria using single root canal irrigants becomes difficult because of the formed biofilm. We aimed to investigate the effectiveness of a combination of C. megacephala maggot extract with the common irrigant, sodium hypochlorite 3%, on the biofilm reduction of E. faecalis. The C. megacephala maggot extract was tested at concentrations 25%, 50%, and 100%, and the combination of each extract concentration with sodium hypochlorite in a volume ratio of 1:1; 1:2, 1:3, 2:1, and 3:1. All treatments were performed three times of replication with incubation time for 1 hour and 3 hours. Antibiofilm effect was measured with crystal violet staining and the optical density reading. Data was analyzed with the Statistical Package for the Social Sciences Statistic Version 22. The least biofilm formation was observed in combination of maggot extract 25% with sodium hypochlorite (2:1) for 1 hour incubation (p=0,05) and combination of maggot extract 25% (1:1) for 3 hours incubation (p=0,000). This combination effectively inhibits the biofilm of E. faecalis. This study identified the protease enzymes in C. megacephala maggot extract and investigated C. megacephala maggot extract's antibiofilm effect combine with the other root canal irrigant.

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in Harvard Style

Hidayati R., Asnani A., Fareza M. and Anjarwati D. (2020). In Vitro Study of Reduction of Oral Enterococcus faecalis Biofilm on Application of Combination of Chrysomya megacephala Maggot Extract and Sodium Hypochlorite.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 98-103. DOI: 10.5220/0010488400980103


in Bibtex Style

@conference{jimc20,
author={Rizka Hidayati and Ari Asnani and Muhamad Salman Fareza and Dwi Utami Anjarwati},
title={In Vitro Study of Reduction of Oral Enterococcus faecalis Biofilm on Application of Combination of Chrysomya megacephala Maggot Extract and Sodium Hypochlorite},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={98-103},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010488400980103},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - In Vitro Study of Reduction of Oral Enterococcus faecalis Biofilm on Application of Combination of Chrysomya megacephala Maggot Extract and Sodium Hypochlorite
SN - 978-989-758-499-2
AU - Hidayati R.
AU - Asnani A.
AU - Fareza M.
AU - Anjarwati D.
PY - 2020
SP - 98
EP - 103
DO - 10.5220/0010488400980103