Expression of HSA-MIR-155-5P and mRNA Suppressor of Cytokine Signalling 1 (SOCS1) on Plasma at Early-stage and Late-stage of Nasopharyngeal Carcinoma

Recita Indraswary, Sofia Mubarika Haryana, Agus Surono

Abstract

Nasopharyngeal carcinoma (NPC) is a head and neck tumor with high prevalence and recurrence rate in Asia. Accurate therapy based on carcinoma pathogenesis at molecular level is urgently needed. Overexpression of hsa-miR-155-5p has been identified in various carcinomas include NPC. In previous in-silico research, hsa-miR-155-5p are known to target mRNA SOCS1, as a transduction signal suppressor for gene transcription activator. This study aimed to analyze different expressions of hsa-miR-155-5p and mRNA SOCS1 on plasma patients at an early and late stage of nasopharynx carcinoma. Hsa-miR-155-5p and mRNA SOCS1 were isolated from blood sample plasma using miRCURY RNA Isolation Kit-Biofluid. cDNA synthesized using cDNA Synthesis kit II, 8-64 rxns running by PCR thermal cycler (Bio-Rad c1000), and Real-time qPCR (Bio-Rad CFX 96) for Hsa-miR-155-5p. mRNA SOCS1 was analyzed by One-Step qRT-PCR using KAPA™ SYBR® kit. Hsa-miR-155-5p expressions change was upregulated 1.13-fold (p value=0.713) on the late-stage compared to the early stage, while for mRNA SOCS1 downregulated 1.1-fold (p value=0.891) on late-stage compared with early stage. Hsa-mir-155-5p was overexpressed on late-stage nasopharynx carcinoma and aligned with mRNA SOCS1 downregulated expression, compared to early stage. Deregulation of -miR-155-5p dan mRNA SOCS1 may play important role in NPC progressivity.

Download


Paper Citation


in Harvard Style

Indraswary R., Haryana S. and Surono A. (2020). Expression of HSA-MIR-155-5P and mRNA Suppressor of Cytokine Signalling 1 (SOCS1) on Plasma at Early-stage and Late-stage of Nasopharyngeal Carcinoma.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 75-80. DOI: 10.5220/0010488000750080


in Bibtex Style

@conference{jimc20,
author={Recita Indraswary and Sofia Mubarika Haryana and Agus Surono},
title={Expression of HSA-MIR-155-5P and mRNA Suppressor of Cytokine Signalling 1 (SOCS1) on Plasma at Early-stage and Late-stage of Nasopharyngeal Carcinoma},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={75-80},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010488000750080},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Expression of HSA-MIR-155-5P and mRNA Suppressor of Cytokine Signalling 1 (SOCS1) on Plasma at Early-stage and Late-stage of Nasopharyngeal Carcinoma
SN - 978-989-758-499-2
AU - Indraswary R.
AU - Haryana S.
AU - Surono A.
PY - 2020
SP - 75
EP - 80
DO - 10.5220/0010488000750080