The Effect of Ethanol Extract of Lingzhi Mushroom (Ganoderma lucidum) on Caspase-3 Expression in Oral Cancer Cells

Irfan Dwiandhono, Fadli Ashar, Arsa Hadiyatama Waskito Aji, Meta Anjay Firmansyah

Abstract

Oral carcinoma is cancer found in the mouth, lips, palate, gingiva, mouth floor, and cheek mucosa. Oral carcinoma is a common cause of death in Indonesia. The development of cancer cells in the oral cavity is affected by the loss of caspase-3 expression. A treatment using lingzhi mushroom is known to increase caspase-3 expression in cancer. This study aimed to know about the effect of ethanol extract of Ganoderma Lucidum on caspase-3 expression in oral carcinoma KB CCL-17. The samples were oral carcinoma KB CCL17 cells with five treated groups 8.49μg/ml (P1), 4.24 μg/ml (P2), 2.2 μg/ml (P3), 11.55 μg/ml (cisplatin), and one control group (K). Caspase-3 expression was analyzed using the immunocytochemistry method by counting the percentage of caspase-3 expression cells. The data were statistically analyzed using One Way ANOVA and Post Hoc LSD. The results of caspase-3 expression on each groups were 5.21 % (P1), 2 % (P2), 0.96 % (P3), 9.67 % (cisplatin) and 0.41 % (K). Ethanol extract of Ganoderma Lucidum increased caspase-3 expression in KB CCL-17 cells along with the increase of the dosage. The dosage of 8.96 μg/ml showed a higher increase of caspase-3 expression than the dosages of 4.24 μg/ml and 2.12 μg/ml. An effect of ethanol extract of lingzhi mushroom on caspase-3 expression in oral carcinoma KB-CCL17. The study using lingzhi mushroom should be more developed to determine the anti-cancer effect through various pathways.

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Paper Citation


in Harvard Style

Dwiandhono I., Ashar F., Waskito Aji A. and Firmansyah M. (2020). The Effect of Ethanol Extract of Lingzhi Mushroom (Ganoderma lucidum) on Caspase-3 Expression in Oral Cancer Cells.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 52-57. DOI: 10.5220/0010487600520057


in Bibtex Style

@conference{jimc20,
author={Irfan Dwiandhono and Fadli Ashar and Arsa Hadiyatama Waskito Aji and Meta Anjay Firmansyah},
title={The Effect of Ethanol Extract of Lingzhi Mushroom (Ganoderma lucidum) on Caspase-3 Expression in Oral Cancer Cells},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={52-57},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010487600520057},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - The Effect of Ethanol Extract of Lingzhi Mushroom (Ganoderma lucidum) on Caspase-3 Expression in Oral Cancer Cells
SN - 978-989-758-499-2
AU - Dwiandhono I.
AU - Ashar F.
AU - Waskito Aji A.
AU - Firmansyah M.
PY - 2020
SP - 52
EP - 57
DO - 10.5220/0010487600520057