Toxicity of Self-nanoemulsifying Drug Delivery System Formulation of Nigella Sativa L. Seed Oil against Adult Danio rerio

Yanti Tri Utami, Isnatin Miladiyah

Abstract

Nigella sativa L. (N. sativa L.) has been used in traditional medicine due to its numerous therapeutic effects, but its oral form has low solubility and has a minor therapeutic effect. The development of self-nanoemulsifying drug delivery system (SNEDDS) formulation for N. sativa L may solve this issue. The pharmacological activity of the SNEDDS formulation of N. sativa L. seed oil extract (NSOE) has been widely explored but its toxicity remains unknown. This study aimed to determine the lethal concentration of NSOE SNEDDS formulation. An experimental study was conducted using adult zebrafish, aged 4–6 months, incubated with NSOE SNEDDS formulation at 500, 250, 125, 62.5, and 31.5 part per million (ppm) concentrations and with non-SNEDDS formulation at 125, 62.5, 31.25, 15.625, and 7.8125 ppm concentrations. The mortality was calculated through macroscopic examination after 24, 48, 72, and 96 hours of exposure. The half-maximal lethal concentration (LC50) of the NSOE SNEDDS and non-SNEDDS formulations was determined using the probit analysis. The LC50 of NSOE SNEDDS formulations was 154.637 + 75.609 ppm and was not significantly different from that of non-SNEDDS (72.358 + 15.253 ppm) (p = 0.138). The toxicity of the SNEDDS formulation of NSOE was comparable to that of the non-SNEDDS.

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Paper Citation


in Harvard Style

Utami Y. and Miladiyah I. (2020). Toxicity of Self-nanoemulsifying Drug Delivery System Formulation of Nigella Sativa L. Seed Oil against Adult Danio rerio.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 35-42. DOI: 10.5220/0010487300350042


in Bibtex Style

@conference{jimc20,
author={Yanti Tri Utami and Isnatin Miladiyah},
title={Toxicity of Self-nanoemulsifying Drug Delivery System Formulation of Nigella Sativa L. Seed Oil against Adult Danio rerio},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={35-42},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010487300350042},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Toxicity of Self-nanoemulsifying Drug Delivery System Formulation of Nigella Sativa L. Seed Oil against Adult Danio rerio
SN - 978-989-758-499-2
AU - Utami Y.
AU - Miladiyah I.
PY - 2020
SP - 35
EP - 42
DO - 10.5220/0010487300350042