Suppression of MnSOD by Andrographolide and its Relation to Oxidative Stress and Viability of Breast Cancer Stem Cells Treated with Repeated Doxorubicin Administration

Angie Tara Rachman, Ayu Suraduhita, Resda Akhra Syahrani, Melva Louisa, Septelia Inawati Wanandi

Abstract

Cancer stem cells (CSCs) are chemoresistance and could be a preferential target for reversing resistance. Doxorubicin is one of the most effective chemotherapy agents, but resistance still occurs. Doxorubicin generates reactive oxygen species (ROS). The increased specific activity of MnSOD has been suggested as one possible mechanism for breast cancer stem cells (BCSCs) to maintain stemness and survival. The study performed to determine the effect of andrographolide on oxidative status and its relation to the viability of BCSCs given repeated doxorubicin. CD24-/CD44+ BCSCs were grown in DMEM/F12 medium with 0,1µM doxorubicin for 14 days, then treated with a combination of 0,1µM doxorubicin and 0,285 mM andrographolide until day 22. MnSOD activity was suppressed on day 4 when BCSCs were still sensitive to doxorubicin treatment. Viability significantly increased on day 20 along with increased MnSOD activity. Andrographolide restored the sensitivity of BCSCs to doxorubicin, which correlated with MnSOD activity but not catalase. There was no change in MDA levels in all days of treatment which means BCSCs can maintain oxidative stress level. Andrographolide supplementation can decrease MnSOD activity but not catalase and is closely related to decreased cell viability with low sensitivity to doxorubicin.

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Paper Citation


in Harvard Style

Rachman A., Suraduhita A., Syahrani R., Louisa M. and Wanandi S. (2020). Suppression of MnSOD by Andrographolide and its Relation to Oxidative Stress and Viability of Breast Cancer Stem Cells Treated with Repeated Doxorubicin Administration.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 28-34. DOI: 10.5220/0010487200280034


in Bibtex Style

@conference{jimc20,
author={Angie Tara Rachman and Ayu Suraduhita and Resda Akhra Syahrani and Melva Louisa and Septelia Inawati Wanandi},
title={Suppression of MnSOD by Andrographolide and its Relation to Oxidative Stress and Viability of Breast Cancer Stem Cells Treated with Repeated Doxorubicin Administration},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={28-34},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010487200280034},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Suppression of MnSOD by Andrographolide and its Relation to Oxidative Stress and Viability of Breast Cancer Stem Cells Treated with Repeated Doxorubicin Administration
SN - 978-989-758-499-2
AU - Rachman A.
AU - Suraduhita A.
AU - Syahrani R.
AU - Louisa M.
AU - Wanandi S.
PY - 2020
SP - 28
EP - 34
DO - 10.5220/0010487200280034