The Role of Fibroblast Proliferation in Wound Healing by Different Plants: An Experimental Study

Marisa Riliani, Indra Kusuma, Abdul Halim, Aliya Muhammad, Akbar Fitrianto, Ida Bagus Eka Narendra

Abstract

Wound healing is a process that consisted of inflammation, proliferation, and migration of fibroblasts. Indigenous people commonly used Manihot esculenta and Ageratum conyzoides L.as bleeding wounds therapy. This study aimed to investigate these two plant extracts on fibroblast proliferation and migration capabilities. Skin fibroblasts were cultured with a medium conditioned for each extract with different concentrations (0.5%, 1%, 2%, 4%), positive and negative control groups. In evaluating proliferation, fibroblasts were incubated by CCK-8 and measured by spectrophotometer expressed as optical density (OD). The evaluation of migration was visualized by scratch assay. The distance between each edge of the scratch was measured using T-scratch software and expressed as the area's closure percentage. There were significant differences in fibroblast proliferation rate in the groups receiving 0.5% Manihot esculenta (p=0.00) and 2% Ageratum conyzoides L. (p=0.00). The migration of fibroblasts was no different, with 0.5% Manihot esculenta (p=0.40) and 2% Ageratum conyzoides L. (p=0.18). Manihot esculenta and Ageratum conyzoides L. could be considered to be used as agents to accelerate wound healing by increasing the fibroblast proliferation. Our findings suggest in vivo studies for tissue regeneration

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Paper Citation


in Harvard Style

Riliani M., Kusuma I., Halim A., Muhammad A., Fitrianto A. and Eka Narendra I. (2020). The Role of Fibroblast Proliferation in Wound Healing by Different Plants: An Experimental Study.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 5-9. DOI: 10.5220/0010486300050009


in Bibtex Style

@conference{jimc20,
author={Marisa Riliani and Indra Kusuma and Abdul Halim and Aliya Muhammad and Akbar Fitrianto and Ida Bagus Eka Narendra},
title={The Role of Fibroblast Proliferation in Wound Healing by Different Plants: An Experimental Study},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={5-9},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010486300050009},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - The Role of Fibroblast Proliferation in Wound Healing by Different Plants: An Experimental Study
SN - 978-989-758-499-2
AU - Riliani M.
AU - Kusuma I.
AU - Halim A.
AU - Muhammad A.
AU - Fitrianto A.
AU - Eka Narendra I.
PY - 2020
SP - 5
EP - 9
DO - 10.5220/0010486300050009