Cell Cycle Inhibition and Apoptosis Induction Activities of n-Hexane Fraction of Cyperus rotundus L. Rhizome

Masfria, Urip Harahap, Denny Satria

Abstract

Background: Breast cancer is one of the leading cause of death and the most common cancer type amongst women in the world after cervic cancer. Objective: To evaluate the cell cycle inhibition and apoptosis induction activities on T47D cell lines of n-hexane fraction (nHF) of Cyperus rotundus L. rhizomes. Methods: Ethanol extract was obtained by maceration method and was fractionated with n-hexane. Cytotoxic activity was examined with MTT assay, and cell cycle inhibition, apoptosis induction and cyclin D1 expression were assessed with flow cytometry method. Results: Cytotoxic activity of nHF was found to have IC 50 of 71.69 ± 0.34 µg/mL, nHF at concentration 35 µg/mL caused accumulation in G 0 -G 1 and S phase accumulation (56.89% and 19.36%), increased early apoptosis (26.30%) and decreased expression of cyclin D1 (26.30%). Conclusions: The results reveal that nHF of Cyperus rotundus L. rhizomes has cell cycle inhibition and apoptosis induction activities. Our further study is to isolation compounds which responsible for these activities.

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Paper Citation


in Harvard Style

Masfria., Harahap U. and Satria D. (2018). Cell Cycle Inhibition and Apoptosis Induction Activities of n-Hexane Fraction of Cyperus rotundus L. Rhizome.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 292-296. DOI: 10.5220/0008361602920296


in Bibtex Style

@conference{bromo18,
author={Masfria and Urip Harahap and Denny Satria},
title={Cell Cycle Inhibition and Apoptosis Induction Activities of n-Hexane Fraction of Cyperus rotundus L. Rhizome},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={292-296},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008361602920296},
isbn={978-989-758-347-6},
}


in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - Cell Cycle Inhibition and Apoptosis Induction Activities of n-Hexane Fraction of Cyperus rotundus L. Rhizome
SN - 978-989-758-347-6
AU - Masfria.
AU - Harahap U.
AU - Satria D.
PY - 2018
SP - 292
EP - 296
DO - 10.5220/0008361602920296