Standardization Raw Material and Ethanolic Extract of
Andrographidis Herba (Andrographis paniculata Nees) from District
of Bogor and Tawangmangu
Sukardiman*, Rakhmawati, Alvi Prabowo , Lusiana Arifianti
Department of Pharmacognosy and phytochemical, Faculty of Pharmacy, Airlangga University, Surabaya, Indonesia
Keyword: Standardization, raw material, ethanolic extract, Andrographis paniculata Nees, Andrographis herbs
Abstract: Sambiloto (Andrographis paniculata Nees) has a potency to be developed in phyto-pharmaceutical product,
especially in this study focused on supporting anti-hyperglycemic research. This requires standardization
can affect both the raw material or extract qualities that have been obtained based on the Indonesian Herbal
Pharmacopeia method. Raw material for this study has been obtained from two traditional drug
development institutions, there are BALITRO from Bogor and BPTO from Tawangmangu.
1 INTRODUCTION
Sambiloto (Andrographis paniculata Nees) is one of
medicinal plant that it has long existed and used as
ingredient of traditional medicine. Sambiloto has
bitter taste, contains flavonoids, tannins, and
saponins (Adelyna, 1999).
Ethanolic extract of sambiloto can restore blood
glucose level into normal in induced-rats, it
indicated as anti-hyperglicemic activity (Yu et al.,
2003). Another study showed that combination
curcumin and andrographolide (3:1) have role in
reducing blood glucose level in rats by 47.945% was
preprandial and 74.159% was postprandial (Cheung
et al., 2001). The study of sambiloto as anti-
hyperglicemic also showed in the mixture formula
such as sambiloto and mahoni reduced blood
glucose level in mice by 250.80 mg/dl (Sukardiman
et al., 2012).
Many studies have proven the efficacy of
sambiloto as anti-diabetic agent and raises potential
to be developed into phyto-pharmaceutical product
as anti-diabetic. Therefore, it is necessary to do
standardization. It can affect quality of both extract
and raw material by methods that have been
established by Ministry of Health through Indonesia
Herbal Pharmacopeia. It is a guarantee for safety,
effectiveness, and especially quality of plant
material, raw material and ethanolic extract that
developed into phyto-pharmaceutical preparation.
2 MATERIAL AND METHODS
2.1. Plant Material
In this research, the raw material was obtained from
two different districts, there are Bogor (BALITRO,
Balai Penelitian Tanaman Obat dan Aromatik) and
Tawangmangu (BPTO, Balai Besar Penelitian dan
Pengembangan Tanaman Obat dan Obat
Tradisional). They prepared in dried raw material.
The ethanolic extract was extracted by maceration
method with 96% ethanol for 24 hours and
performed up to three times (Pholphana et al., 2004).
The mixture extract was concentrate using rotary
evaporator.
284
Sukardiman, ., Rakhmawati, ., Prabowo, A. and Arifianti, L.
Standardization Raw Material and Ethanolic Extract of Andrographidis Herba (Andrographis paniculata Nees) from District of Bogor and Tawangmangu.
DOI: 10.5220/0008361402840287
In Proceedings of BROMO Conference (BROMO 2018), pages 284-287
ISBN: 978-989-758-347-6
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