In Vitro Antibacterial Activity Test of Jackfruit (Artocarpus heterophyllus Lam.) Leaf Extract against Methicillin-Resistant Staphylococcus aureus (MRSA)

Ika Ningsih, Dyah Ayu Rosalinda, Ariyani Kiranasari, Beti Ernawati Dewi, Fithriyah Sjatha

Abstract

Infectious diseases are still a public health problem in Indonesia. Methicillin Resistant Staphylococcus aureus (MRSA) is one of bacteria causing infections that is a concern because of the nature of resistance to various beta-lactam class of antibiotics. Therefore, it is necessary to find an alternative antibiotic for the treatment of MRSA infections. This study aims to determine the antibacterial activity of jackfruit (Artocarpus heterophyllus Lam.) leaf extract against MRSA by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) conducted using in-vitro test with broth macrodilution method. Jackfruit (Artocarpus heterophyllus Lam.) leaf extract were used in half serial concentration of 1280 μg/ml to 0,625 μg/ml. MIC of jackfruit leaf extract against MRSA was found at concentration of 320 μg/ml which showed by a clear solution in Brain Heart Infusion media. Meanwhile, MBC value of jackfruit leaf extract against MRSA was found at concentration of 1280 μg/ml showed by the absence of MRSA colony growth Mueller-Hinton agar. From this study showed that jackfruit (Artocarpus heterophyllus Lam.) leaf extract has anti-bacterial activity against MRSA in-vitro.

Paper Citation

in Harvard Style

Ningsih I., Rosalinda D., Kiranasari A., Dewi B. and Sjatha F. (2018). In Vitro Antibacterial Activity Test of Jackfruit (Artocarpus heterophyllus Lam.) Leaf Extract against Methicillin-Resistant Staphylococcus aureus (MRSA).In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 245-249. DOI: 10.5220/0008360702450249

in Bibtex Style

@conference{bromo18,
author={Ika Ningsih and Dyah Ayu Rosalinda and Ariyani Kiranasari and Beti Ernawati Dewi and Fithriyah Sjatha},
title={In Vitro Antibacterial Activity Test of Jackfruit (Artocarpus heterophyllus Lam.) Leaf Extract against Methicillin-Resistant Staphylococcus aureus (MRSA)},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={245-249},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008360702450249},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - In Vitro Antibacterial Activity Test of Jackfruit (Artocarpus heterophyllus Lam.) Leaf Extract against Methicillin-Resistant Staphylococcus aureus (MRSA)
SN - 978-989-758-347-6
AU - Ningsih I.
AU - Rosalinda D.
AU - Kiranasari A.
AU - Dewi B.
AU - Sjatha F.
PY - 2018
SP - 245
EP - 249
DO - 10.5220/0008360702450249