Separation of Ethyl Acetate Fraction of Mengkudu Fruit (Morinda citrifolia Linn.) and Its Antidiabetic Activity by Glucose Tolerance Method on Mice

Shintia Lintang Charisma, Yasmiwar Susilawati, Ahmad Muhtadi

Abstract

Mengkudu (Morinda citrifolia Linn.) is one of the medicinal plant that contains alkaloids, carbohydrates, flavonoids, glycosides, phenols, proteins, amino acids, saponins, steroids, tannins, antraquinone and terpenoids. Traditionally, mengkudu (M. citrifolia Linn.) fruit has been used for antidiabetic effect. Previous research showed that ethyl acetate fraction gave significant activity (54.29 %) at dose 1200 mg/kg body weight on male wistar rats. The aims of this study were to separate ethyl acetate fraction from mengkudu fruit by Vacuum Liquid Chromatography method and to study antidiabetic activities on male mice by glucose tolerance method. This research used separation guided by activity method which ethyl acetate fraction separated by Vacuum Liquid Chromatography and gave five subfractions (Ds II-A, Ds II-B, Ds II-C, Ds II-D and Ds II-E). The five subfractions then tested the antidiabetic activity at a dose of 150 mg/kg body weight on male mice by glucose tolerance method. Blood glucose level measured at 30, 50, 90, 120 and 150 minutes after administration of subfractions. Ethyl acetate subfractions from mengkudu fruit (Ds II-A, Ds II-B, Ds II-C, Ds II-D and Ds II-E) showed antidiabetic activity with percentage of reduction relative blood glucose levels 150 minutes after sample administration were 39.11%, 52.85%, 35.31% 43.55%, and 33.78% respectively. These research showed that Ds-II-B subfraction indicate the highest antidiabetic activity by glucose tolerance method. Rutin, quercetin and scopoletin compounds indicate antidiabetic activity from ethyl acetate subfractions of mengkudu fruit.

Paper Citation

in Harvard Style

Charisma S., Susilawati Y. and Muhtadi A. (2018). Separation of Ethyl Acetate Fraction of Mengkudu Fruit (Morinda citrifolia Linn.) and Its Antidiabetic Activity by Glucose Tolerance Method on Mice.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 218-223. DOI: 10.5220/0008360302180223

in Bibtex Style

@conference{bromo18,
author={Shintia Lintang Charisma and Yasmiwar Susilawati and Ahmad Muhtadi},
title={Separation of Ethyl Acetate Fraction of Mengkudu Fruit (Morinda citrifolia Linn.) and Its Antidiabetic Activity by Glucose Tolerance Method on Mice},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={218-223},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008360302180223},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - Separation of Ethyl Acetate Fraction of Mengkudu Fruit (Morinda citrifolia Linn.) and Its Antidiabetic Activity by Glucose Tolerance Method on Mice
SN - 978-989-758-347-6
AU - Charisma S.
AU - Susilawati Y.
AU - Muhtadi A.
PY - 2018
SP - 218
EP - 223
DO - 10.5220/0008360302180223