Evaluation of Antioxidant and Cytotoxic Activities of Vernonia amygdalina Del. Leaves

Poppy Anjelisa Zaitun Hasibuan, Urip Harahap, Panal Sitorus, Denny Satria

Abstract

The excessive production of oxygen free radicals and the unbalanced mechanism of antioxidant protection results in the onset of many diseases such as breast cancer. Antioxidant activity was determined by 1-1-diphenyl-2- picrylhydrazil (DPPH) method and cytotoxic activity was determined with MTT method towards T47D cell line. Antioxidant activity from n-hexane, ethylacetate, ethanol fractions and quercetine as positive control with DPPH assay measured as IC 50 were 297.33 ± 0.46; 177.99 ± 0.32; 37.92 ± 1.03 and 2.32 ± 0.01 µg/mL respectively. Cytotoxic activity from n-hexane, ethylacetate, ethanol fractions and doxorubicin as positive control with MTT assay measured as IC 50 were 327.89 ± 1.13; 64.92 ± 0.72; 1591.75 ± 37.05 and 1.82 ± 0.05 µg/mL respectively. The results reveal that fractions of Vernonia amygdalina Del. leaves have antioxidant and cytotoxic activities. Our further study is to asses anticancer mechanism of Vernonia amygdalina Del. leaves.

Paper Citation

in Harvard Style

Hasibuan P., Harahap U., Sitorus P. and Satria D. (2018). Evaluation of Antioxidant and Cytotoxic Activities of Vernonia amygdalina Del. Leaves.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 214-217. DOI: 10.5220/0008360202140217

in Bibtex Style

@conference{bromo18,
author={Poppy Anjelisa Zaitun Hasibuan and Urip Harahap and Panal Sitorus and Denny Satria},
title={Evaluation of Antioxidant and Cytotoxic Activities of Vernonia amygdalina Del. Leaves},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={214-217},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008360202140217},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - Evaluation of Antioxidant and Cytotoxic Activities of Vernonia amygdalina Del. Leaves
SN - 978-989-758-347-6
AU - Hasibuan P.
AU - Harahap U.
AU - Sitorus P.
AU - Satria D.
PY - 2018
SP - 214
EP - 217
DO - 10.5220/0008360202140217