Mechanism of Antimicrobial Action of Ocimum Basilicum Essential Oil Against Nosocomial Bacteria

Fadli Asmani, Kiran Chanabasappa Nilugal, Sherilyn Fenn Karel, Santosh Fattepur, May Florence Dela Cruz Bacayo, Wong Charng Choon, Mohamed Rasny, Eddy Yusuf

Abstract

BACKGROUND: Nosocomial infection is a major concern in the healthcare sector. Ocimum basilicum essential oil is one of the main essential oil being investigated for its antimicrobial activities. OBJECTIVES: This study was conducted to determine the effect of Ocimum basilicum essential oil on bacterial cell growth and cell membrane integrity. METHOD: The O. basilicum essential oil (EO) was purchased from Natur Aromatherapy and Wellness and different concentrations of the essential oil (0.092 – 1.470 g/ml) was prepared using 10% DMSO. Disc diffusion test and MIC using broth dilution for Escherichia coli and Staphylococcus aureus, with Gentamicin as positive control were conducted. 24-hour growth kinetic analysis of the bacteriaand their cell membrane integrity were investigated by measuring the absorbance value. RESULT: The zone of inhibition by the essential oil was greater in S.aureus compared to E.coli and the MIC was 0.368g/ml and 0.735g/ml respectively. EO concentrations at 1.47 g/ml and 0.735 g/ml are the only one showed effective inhibition on the growth of the bacteria. The EO was more effective in inhibiting the growth of S.aureusthan E.coli. The absorbance of the bacterial cell constituents increased from the negative control, 2 x MIC, MIC and Gentamicin in both bacteria. CONCLUSION:Ocimum basilicum essential oil exert its antimicrobial action by affecting bacterial growth and acting on cell membrane integrity of the bacteria.

Paper Citation

in Harvard Style

Asmani F., Nilugal K., Karel S., Fattepur S., Bacayo M., Choon W., Rasny M. and Yusuf E. (2018). Mechanism of Antimicrobial Action of Ocimum Basilicum Essential Oil Against Nosocomial Bacteria.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 202-208. DOI: 10.5220/0008360002020208

in Bibtex Style

@conference{bromo18,
author={Fadli Asmani and Kiran Chanabasappa Nilugal and Sherilyn Fenn Karel and Santosh Fattepur and May Florence Dela Cruz Bacayo and Wong Charng Choon and Mohamed Rasny and Eddy Yusuf},
title={Mechanism of Antimicrobial Action of Ocimum Basilicum Essential Oil Against Nosocomial Bacteria},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={202-208},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008360002020208},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - Mechanism of Antimicrobial Action of Ocimum Basilicum Essential Oil Against Nosocomial Bacteria
SN - 978-989-758-347-6
AU - Asmani F.
AU - Nilugal K.
AU - Karel S.
AU - Fattepur S.
AU - Bacayo M.
AU - Choon W.
AU - Rasny M.
AU - Yusuf E.
PY - 2018
SP - 202
EP - 208
DO - 10.5220/0008360002020208