The Antibacterial Activity of Isolated Flavonoid Fractions from Ethanol Ethanolic Peel of Citrus Sinensis (Valencia Orange) with Citrus Limon (Lemon) against Staphylococcus Aureus and Pseudomonas Aeruginosa

Nik Nur Shamiha N. D., Ghayatery Nagatamby, Jiyauddin Khan, Fadli Asmani, Eddy Yusuf

Abstract

Background: Skin and soft tissue infections (SSTIs) are most common infections encountered by all physicians. Eventhough pharmacological industries have produced a number of new antibiotics, resistance to these drugs by microorganisms has increased. Objective: The aim of this study is to evaluate the antibacterial activity of isolated flavonoid fractions from ethanolic peel extract of C.sinensis (Orange), C.limon (Lemon) and its combination against S.aureus and P.aeruginosa. Methods: The ethanolic peel extract of both plant were screened for phytochemical identification of flavonoid by lead acetate test and shinoda test. The extract of both plants were evaluated for preliminary antibacterial activity using disk diffusion method. Thin-layer chromatography and column chromatography was performed to isolate flavonoids. Isolated flavonoids were subjected to determination of minimum inhibitory concentration and antibacterial assay by disk diffusion method. Results: Isolated flavonoids of C.sinensis (17mm, 8mm), C.limon (20mm, 9mm) and its combination (24mm, 14mm) produced antibacterial activity that is comparable to the Ciprofloxacin disc (30mm, 9mm) against S.aureus and P.aeruginosa respectively. Thus, these results suggested that C.limon produced a better antibacterial activity against both bacteria compared to C.sinensis. However, the combination of both plants isolated flavonoid fractions produced much better antibacterial activity against S.aureus and P.aeruginosa in comparison with individual flavonoid fractions of both plants. Conclusion: Therefore, Citrus fruits peels that is being as primary waste in juicing industries can be further developed as marketable natural source of antibiotic as a treatment of SSTIs.

Paper Citation

in Harvard Style

Shamiha N. D. N., Nagatamby G., Khan J., Asmani F. and Yusuf E. (2018). The Antibacterial Activity of Isolated Flavonoid Fractions from Ethanol Ethanolic Peel of Citrus Sinensis (Valencia Orange) with Citrus Limon (Lemon) against Staphylococcus Aureus and Pseudomonas Aeruginosa.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 194-197. DOI: 10.5220/0008359801940197

in Bibtex Style

@conference{bromo18,
author={Nik Nur Shamiha N. D. and Ghayatery Nagatamby and Jiyauddin Khan and Fadli Asmani and Eddy Yusuf},
title={The Antibacterial Activity of Isolated Flavonoid Fractions from Ethanol Ethanolic Peel of Citrus Sinensis (Valencia Orange) with Citrus Limon (Lemon) against Staphylococcus Aureus and Pseudomonas Aeruginosa},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={194-197},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008359801940197},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - The Antibacterial Activity of Isolated Flavonoid Fractions from Ethanol Ethanolic Peel of Citrus Sinensis (Valencia Orange) with Citrus Limon (Lemon) against Staphylococcus Aureus and Pseudomonas Aeruginosa
SN - 978-989-758-347-6
AU - Shamiha N. D. N.
AU - Nagatamby G.
AU - Khan J.
AU - Asmani F.
AU - Yusuf E.
PY - 2018
SP - 194
EP - 197
DO - 10.5220/0008359801940197